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Literature summary for 4.2.1.135 extracted from

  • Cho, A.; Lee, S.; Kim, B.; Ahn, J.
    Biosynthesis of three N-acetylaminosugar-conjugated flavonoids using engineered Escherichia coli (2016), Microb. Cell Fact., 15, 182 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.135

Cloned(Commentary)

Cloned (Comment) Organism
gene Pdeg or pglF, recombinant expression in Escherichia coli, coexpression with Preq (UDP-4-reductase), UDP-GlcNAc 6-DH (UDP-N-acetylglucosamine 6-dehydrogenase), and UXNAcS (UDP-N-acetylxylosamine synthase), leading to biosynthesis of flavonoid glycosides in the engineered Escherichia coli strain, overview Bacillus cereus

Protein Variants

Protein Variants Comment Organism
additional information four genes, Pdeg (UDP-N-acetylglucosamine C4,6-dehydratase), Preq (UDP-4-reductase), UDP-GlcNAc 6-DH (UDP-N-acetylglucosamine 6-dehydrogenase), and UXNAcS (UDP-N-acetylxylosamine synthase), are employed to synthesize UDP-quinovosamine, UDP-N-acetylglucosaminuronic acid, and UDP-N-acetylxylosamine in Escherichia coli. Escherichia coli nucleotide sugar biosynthetic pathway is engineered to increase the pool of substrate for the target nucleotide sugars. Uridine diphosphate dependent glycosyltransferase (UGT) is also selected and introduced into Escherichia coli. High levels of three novel flavonoid glycosides are obtained in the engineered mutant with 158.3 mg/l quercetin 3-O-(N-acetyl) quinovosamine, 172.5 mg/l luteolin 7-O-(N-acetyl)glucosaminuronic acid, and 160.8 mg/l quercetin 3-O-(N-acetyl)xylosamine Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine Bacillus cereus
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 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
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 ?
UDP-N-acetyl-alpha-D-glucosamine Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711
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 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
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-
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Bacillus cereus Q81A42
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Bacillus cereus ATCC 14579
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-
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Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 Q81A42
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine
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 Bacillus cereus UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
-
 ?
UDP-N-acetyl-alpha-D-glucosamine
-
 Bacillus cereus ATCC 14579 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
-
 ?
UDP-N-acetyl-alpha-D-glucosamine
-
 Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
-
 ?

Synonyms

Synonyms Comment Organism
BC_3750
-
 Bacillus cereus
Pdeg
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 Bacillus cereus
UDP-N-acetylglucosamine C4,6-dehydratase
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 Bacillus cereus

General Information

General Information Comment Organism
metabolism the enzme is involved in the biosynthesis of Bacillus glycans and N-acetylquinovosamine (2-acetamido-2,6-di-deoxy-D-glucose, QuiNAc) Bacillus cereus
physiological function gene Pdeg encodes an UDP-N-acetylglucosamine C4,6-dehydratase that converts UDP-GlcNAc to UDP-4-keto-4,6-D-deoxy-GlcNAc (UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose). The product is then catalyzed by Preq-encoded UDP-4-reductase that converts UDP-4-keto-4,6-d-deoxy-GlcNAc to UDP-N-acetylquinovosamine Bacillus cereus