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Literature summary for 4.2.1.135 extracted from

  • Olivier, N.B.; Chen, M.M.; Behr, J.R.; Imperiali, B.
    In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system (2006), Biochemistry, 45, 13659-13669.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
full-length PglF is overexpressed as a GST-fusion protein Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
DELTA1-129 a truncated form of pglF coding for residues M130-V590 is generated omitting the transmembrane domain which expressed well in Escherichia coli. Substrate turnover is not observed in a reaction coupling the deletion construct DELTA1-129 and PglE suggesting the involvement of the PglF transmembrane domain in preventing the inhibitory reaction Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
UDP-N-acetyl-alpha-D-glucosamine pH 7.7, 37°C, full-length GST-PglF Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
SDS-PAGE, GST-PglF Campylobacter jejuni

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in the presence of detergent Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine PglF performs a cofactor-dependent hydride transfer from the C4 position of UDP-GlcNAc to C6, in conjunction with the elimination of water across the glucosyl C5 and C6 bond Campylobacter jejuni UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O
-
?

Synonyms

Synonyms Comment Organism
Cj1120c
-
Campylobacter jejuni
PglF
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.118
-
UDP-N-acetyl-alpha-D-glucosamine pH 7.7, 37°C, full-length GST-PglF Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Campylobacter jejuni

Cofactor

Cofactor Comment Organism Structure
NAD+ catalytic activity by GST-PglF is enhanced in the presence of additional NAD+ but not NADP+ Campylobacter jejuni