Cloned (Comment) | Organism |
---|---|
full-length PglF is overexpressed as a GST-fusion protein | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-129 | a truncated form of pglF coding for residues M130-V590 is generated omitting the transmembrane domain which expressed well in Escherichia coli. Substrate turnover is not observed in a reaction coupling the deletion construct DELTA1-129 and PglE suggesting the involvement of the PglF transmembrane domain in preventing the inhibitory reaction | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 7.7, 37°C, full-length GST-PglF | Campylobacter jejuni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
SDS-PAGE, GST-PglF | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified in the presence of detergent | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine | PglF performs a cofactor-dependent hydride transfer from the C4 position of UDP-GlcNAc to C6, in conjunction with the elimination of water across the glucosyl C5 and C6 bond | Campylobacter jejuni | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cj1120c | - |
Campylobacter jejuni |
PglF | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 7.7, 37°C, full-length GST-PglF | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | catalytic activity by GST-PglF is enhanced in the presence of additional NAD+ but not NADP+ | Campylobacter jejuni |