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Literature summary for 4.2.1.134 extracted from

  • Ianiri, G.; Abhyankar, R.; Kihara, A.; Idnurm, A.
    Phs1 and the synthesis of very long chain fatty acids are required for ballistospore formation (2014), PLoS ONE, 9, e105147 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene phs1, encoding 3-hydroxyacyl-CoA dehydratase required for the third step in very long chain fatty acid biosynthesis, the Sporobolomyces sp. PHS1 gene complements the essential functions of the Saccharomyces cerevisiae phs1 loss-of-function mutant strain phs1::KanMX/PHS1, transfection using the Agrobacterium tumefaciens system, lipid analysis of the strains Sporobolomyces sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of a Sporobolomyces phs1 mutant strain GI209 by T-DNA insertion. The mutant has less dehydratase activity and a reduction in very long chain fatty acids compared to wild-type. The phs1 mutation in Sporobolomyces causes reduction in 3-hydroxyacyl-CoA dehydratase activity and alters the lipid profiles of the cells. The mutant strain also exhibits sensitivity to cell wall stress agents and loss of shooting due to a delay in ballistospore formation Sporobolomyces sp.

Organism

Organism UniProt Comment Textmining
Sporobolomyces sp. V9LTL9
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Sporobolomyces sp. IAM13481 V9LTL9
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Synonyms

Synonyms Comment Organism
PHS1
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Sporobolomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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in vivo assay at Sporobolomyces sp.

General Information

General Information Comment Organism
malfunction an enzyme mutant strain exhibits sensitivity to cell wall stress agents and loss of shooting due to a delay in ballistospore formation. The phs1 deletion mutation in Sporobolomyces causes reduction in 3-hydroxyacyl-CoA dehydratase activity and alters the lipid profiles of the cells, mirror mutant phenotype, overview. Stress phenotypes of the phs1 mutant of Sporobolomyces suggest that defects in other cellular processes can impair the formation of spores, overview Sporobolomyces sp.
physiological function the 3-hydroxyacyl-CoA dehydratase is required for the third step in very long chain fatty acid biosynthesis. The role of Phs1 in spore dissemination may be primarily in cellular integrity Sporobolomyces sp.