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Literature summary for 4.2.1.134 extracted from

  • Ikeda, M.; Kanao, Y.; Yamanaka, M.; Sakuraba, H.; Mizutani, Y.; Igarashi, Y.; Kihara, A.
    Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis (2008), FEBS Lett., 582, 2435-2440.
    View publication on PubMed
Search for more literature for 4.2.1.134

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain SAY32 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
 3-hydroxypalmitoyl-CoA isoform HCAD4, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37°C Homo sapiens
0.0336
-
 3-hydroxypalmitoyl-CoA isoform HCAD1, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37°C Homo sapiens
0.0495
-
 3-hydroxypalmitoyl-CoA isoform HCAD3, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37°C Homo sapiens
0.1217
-
 3-hydroxypalmitoyl-CoA isoform HCAD2, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54900
-
 x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence Homo sapiens
55800
-
 x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence Homo sapiens
59800
-
 x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence Homo sapiens
70600
-
 x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypalmitoyl-CoA
-
 Homo sapiens 2,3-trans-hexadecenoyl-CoA + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence Homo sapiens
? x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence Homo sapiens
? x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence Homo sapiens
? x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
3-hydroxyacyl-CoA dehydratase
-
 Homo sapiens
HACD1 isoform Homo sapiens
HACD2 isoform Homo sapiens
HACD3 isoform Homo sapiens
HACD4 isoform Homo sapiens