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Literature summary for 4.2.1.131 extracted from
- Heterologous expression, purification, and enzymatic characterization of the acyclic carotenoid 1,2-hydratase from Rubrivivax gelatinosus (2003), Arch. Biochem. Biophys., 414, 51-58.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rubrivivax gelatinosus |
| expression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0078 | - |
spheroidene | pH 7.8, 28°C | Rubrivivax gelatinosus |  |
| 0.0221 | - |
lycopene | pH 7.8, 28°C | Rhodobacter capsulatus | |
| 0.0245 | - |
Neurosporene | pH 7.8, 28°C | Rhodobacter capsulatus | |
| 0.0269 | - |
lycopene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
| 0.0368 | - |
1-hydroxyneurosporene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
| 0.0385 | - |
Neurosporene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 44000, SDS-PAGE | Rubrivivax gelatinosus |
| 44000 | - |
x * 44000, SDS-PAGE | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
- |
- |
| Rubrivivax gelatinosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Rubrivivax gelatinosus |
| recombinant protein | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-3,4-didehydrolycopene + H2O | conversion rate 20% | Rubrivivax gelatinosus | ? | - |
? | |
| 1-hydroxylycopene + H2O | - |
Rubrivivax gelatinosus | 1,1'-dihydroxylycopene | - |
? | |
| 1-hydroxyneurosporene + H2O | - |
Rubrivivax gelatinosus | 1,1'-dihydroxyneurosporene | - |
? | |
| demethylspheroidene + H2O | conversion rate 14% | Rubrivivax gelatinosus | ? | - |
? | |
| lycopene + H2O | conversion rate 28%, final product | Rhodobacter capsulatus | 1-hydroxylycopene | - |
? | |
| lycopene + H2O | conversion rate 66% | Rubrivivax gelatinosus | 1-hydroxylycopene | - |
? | |
| additional information | no substrate: 1-methoxylycopene | Rubrivivax gelatinosus | ? | - |
? | |
| additional information | no substrate: 1-methoxylycopene. Enzyme is extremely poor in converting 1-hydroxy-carotenoids | Rhodobacter capsulatus | ? | - |
? | |
| neurosporene + H2O | conversion rate 20%, final product | Rhodobacter capsulatus | 1-hydroxyneurosporene | - |
? | |
| neurosporene + H2O | conversion rate 63% | Rubrivivax gelatinosus | 1-hydroxyneurosporene | - |
? | |
| spheroidene + H2O | conversion rate 12% | Rubrivivax gelatinosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Rubrivivax gelatinosus |
| ? | x * 44000, SDS-PAGE | Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor required | Rubrivivax gelatinosus | |
| additional information | no cofactor required | Rhodobacter capsulatus |
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