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Literature summary for 4.2.1.130 extracted from
- Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe (2014), BMC Evol. Biol., 14, 86.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | - |
Schizosaccharomyces pombe | |
| bovine serum albumin | - |
Saccharomyces cerevisiae | |
| additional information | the enzyme shows GSH-independent glyoxalase activity | Schizosaccharomyces pombe | |
| additional information | the enzyme shows GSH-independent glyoxalase activity | Saccharomyces cerevisiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally GFP-tagged SpDJ-1, expression of wild-type and mutant SpDj-1 proteins in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview | Schizosaccharomyces pombe |
| gene hsp3101 encoding a Hsp31 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells | Schizosaccharomyces pombe |
| gene hsp3101, expression of C-terminally GFP-tagged HSP31 proteins, expression of HSP3101 protein in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview | Schizosaccharomyces pombe |
| gene hsp3102 encoding a Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells | Schizosaccharomyces pombe |
| gene hsp3106 encoding DJ-1 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis | Schizosaccharomyces pombe |
| gene ScHSP31 encoding Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells | Saccharomyces cerevisiae |
| gene ScHSP31, expression in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview. Overexpression of ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C111A | site-directed mutagenesis, catalytically inactive mutant | Schizosaccharomyces pombe |
| E16A | site-directed mutagenesis, catalytically inactive mutant | Schizosaccharomyces pombe |
| H130A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Schizosaccharomyces pombe |
| additional information | overexpression of Saccharomyces cerevisiae gene ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe | Schizosaccharomyces pombe |
| additional information | overexpression of Saccharomyces cerevisiae ScHSP31 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe | Saccharomyces cerevisiae |
| additional information | overexpression of Schizosaccharomyces pombe hsp3101 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe | Schizosaccharomyces pombe |
| additional information | overexpression of Schizosaccharomyces pombe hsp3102 confers methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1 deletion cells of Schizosaccharomyces pombe | Schizosaccharomyces pombe |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
methylglyoxal | recombinant ScHsp31, pH and temperature not specified in the publication | Saccharomyces cerevisiae |  |
| 10.8 | - |
methylglyoxal | recombinant wild-type SpDJ-1, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 31.1 | - |
methylglyoxal | recombinant Hsp3101, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 37.3 | - |
methylglyoxal | recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 58 | - |
methylglyoxal | recombinant Hsp3102, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Schizosaccharomyces pombe | 5737 | - |
| additional information | recombinant GFP-tagged Hsp3102 is only localized in the cytoplasm, not in the nucleus | Schizosaccharomyces pombe | - |
- |
| nucleus | - |
Schizosaccharomyces pombe | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate | Schizosaccharomyces pombe | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | Saccharomyces cerevisiae | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | Schizosaccharomyces pombe 972 | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | 2-oxopropanal + H2O | - |
? | |
| acetate | Schizosaccharomyces pombe | - |
2-oxoethanal + H2O | - |
? | |
| acetate | Saccharomyces cerevisiae | - |
2-oxoethanal + H2O | - |
? | |
| acetate | Schizosaccharomyces pombe 972 | - |
2-oxoethanal + H2O | - |
? | |
| methylglyoxal + H2O | Schizosaccharomyces pombe | - |
(R)-lactate | - |
? | |
| methylglyoxal + H2O | Saccharomyces cerevisiae | - |
(R)-lactate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q04432 | - |
- |
| Saccharomyces cerevisiae | Q04432 | gene ScHSP31 | - |
| Schizosaccharomyces pombe | - |
genes hsp3101 and hsp3102 | - |
| Schizosaccharomyces pombe | O74914 | - |
- |
| Schizosaccharomyces pombe | Q09675 | - |
- |
| Schizosaccharomyces pombe | Q10356 | - |
- |
| Schizosaccharomyces pombe 972 | O74914 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate | - |
Schizosaccharomyces pombe | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | - |
Saccharomyces cerevisiae | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | Schizosaccharomyces pombe | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | Saccharomyces cerevisiae | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | - |
Schizosaccharomyces pombe 972 | 2-oxopropanal + H2O | - |
? | |
| (R)-lactate | glyoxalase III (GLO3) converts methylglyoxal directly into D-lactate in a single step, independent of glutathione GSH | Schizosaccharomyces pombe 972 | 2-oxopropanal + H2O | - |
? | |
| acetate | - |
Schizosaccharomyces pombe | 2-oxoethanal + H2O | - |
? | |
| acetate | - |
Saccharomyces cerevisiae | 2-oxoethanal + H2O | - |
? | |
| acetate | - |
Schizosaccharomyces pombe 972 | 2-oxoethanal + H2O | - |
? | |
| methylglyoxal + H2O | - |
Schizosaccharomyces pombe | (R)-lactate | - |
? | |
| methylglyoxal + H2O | - |
Saccharomyces cerevisiae | (R)-lactate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DJ-1 | - |
Schizosaccharomyces pombe |
| GLO3 | - |
Schizosaccharomyces pombe |
| GLO3 | - |
Saccharomyces cerevisiae |
| glutathione-independent glyoxalase III | - |
Schizosaccharomyces pombe |
| glutathione-independent glyoxalase III | - |
Saccharomyces cerevisiae |
| glyoxalase III | - |
Schizosaccharomyces pombe |
| glyoxalase III | - |
Saccharomyces cerevisiae |
| GSH-independent glyoxalase III | - |
Schizosaccharomyces pombe |
| GSH-independent glyoxalase III | - |
Saccharomyces cerevisiae |
| Hsp31 | - |
Saccharomyces cerevisiae |
| Hsp3101 | - |
Schizosaccharomyces pombe |
| Hsp3102 | - |
Schizosaccharomyces pombe |
| hsp3106 | - |
Schizosaccharomyces pombe |
| SpDJ-1 | - |
Schizosaccharomyces pombe |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.518 | - |
methylglyoxal | recombinant Hsp3101, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 0.967 | - |
methylglyoxal | recombinant Hsp3102, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 1.09 | - |
methylglyoxal | recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 1.25 | - |
methylglyoxal | recombinant ScHsp31, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
| 1.43 | - |
methylglyoxal | recombinant wild-type SpDJ-1, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme shows GSH-independent glyoxalase activity | Schizosaccharomyces pombe | |
| additional information | the enzyme shows GSH-independent glyoxalase activity | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins in fungi, overview | Schizosaccharomyces pombe |
| evolution | detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins in fungi, overview | Saccharomyces cerevisiae |
| evolution | fungal Hsp31 proteins are the major glyoxalases III GLO3 that may have some role in protecting cells from reactive carbonyl species toxicity in fungi. The GLO3 activity of Hsp31 proteins may have evolved independently from GLO3 activity of DJ-1 proteins. DJ-1 and Hsp31 proteins belong to different subfamilies of the DJ-1/Hsp31/PfpI superfamily, which encompasses a wide variety of functionally diverse proteins, detailed phylogenetic analysis of DJ-1 and Hsp31 proteins, distribution of Hsp31 proteins in fungi, overview | Schizosaccharomyces pombe |
| evolution | fungal Hsp31 proteins are the major glyoxalases III GLO3 that may have some role in protecting cells from reactive carbonyl species toxicity in fungi. The GLO3 activity of Hsp31 proteins may have evolved independently from GLO3 activity of DJ-1 proteins. DJ-1 and Hsp31 proteins belong to different subfamilies of the DJ-1/Hsp31/PfpI superfamily, which encompasses a wide variety of functionally diverse proteins, detailed phylogenetic analysis of DJ-1 and Hsp31 proteins, distribution of Hsp31 proteins in fungi, overview | Saccharomyces cerevisiae |
| additional information | two Schizosaccharomyces pombe Hsp31 proteins (Hsp3101 and Hsp3102) and one Saccharomyces cerevisiae Hsp31 protein (ScHsp31) display significantly higher in vitro GLO3 activity than Schizosaccharomyces pombe DJ-1 (SpDJ-1) | Schizosaccharomyces pombe |
| additional information | two Schizosaccharomyces pombe Hsp31 proteins (Hsp3101 and Hsp3102) and one Saccharomyces cerevisiae Hsp31 protein (ScHsp31) display significantly higher in vitro GLO3 activity than Schizosaccharomyces pombe DJ-1 (SpDJ-1) | Saccharomyces cerevisiae |
| additional information | both DJ-1 and Hsp31 proteins possess the Glu-Cys-His catalytic triad, Glu16-Cys111-His130 in SpDJ-1 | Schizosaccharomyces pombe |
| additional information | both DJ-1 and Hsp31 proteins possess the Glu-Cys-His catalytic triad, Glu16-Cys111-His130 in SpDJ-1 | Saccharomyces cerevisiae |
| physiological function | glyoxalase III is responsible for the detoxification of reactive carbonyl species, e.g. methylglyoxal and glyoxal, via the GSH-independent pathway, overview | Schizosaccharomyces pombe |
| physiological function | glyoxalase III is responsible for the detoxification of reactive carbonyl species, e.g. methylglyoxal and glyoxal, via the GSH-independent pathway, overview | Saccharomyces cerevisiae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
methylglyoxal | recombinant mutant SpDJ-1 H130A, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 0.132 | - |
methylglyoxal | recombinant wild-type SpDJ-1, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 0.35 | - |
methylglyoxal | recombinant Hsp3102, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 0.383 | - |
methylglyoxal | recombinant Hsp3101, pH and temperature not specified in the publication | Schizosaccharomyces pombe | |
| 0.85 | - |
methylglyoxal | recombinant ScHsp31, pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
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