Literature summary for 4.2.1.125 extracted from

Huang, Z.; Lin, J.; Cheng, Z.; Xu, M.; Huang, X.; Yang, Z.; Zheng, J.
Production of dammarane-type sapogenins in rice by expressing the dammarenediol-II synthase gene from Panax ginseng C.A. Mey (2015), Plant Sci., 239, 106-114 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene DDS, functional recombinant overexpression in Oryza sativa cv. Shuhui 527 plants using the Agrbacterium tumefaciens transfection method, transfer-DNA (T-DNA) insertion sites in homozygous lines of the T2 generation are determined by using high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR), quantitative RT-PCR expression analysis	Panax ginseng

Protein Variants

Protein Variants	Comment	Organism
additional information	establishment of dammarane-type ginsenosides synthesis from 2,3-oxidosqualene in Oryza sativa by expression of dammarenediol-II synthase from Panax ginseng resulting in a dammarane-type sapogenin 20(S)-protopanaxadiol (PPD) content of 0.35-0.59 mg/gdw and a dammarane-type sapogenin 20(S)-protopanaxatriol (PPT) content of 0.23-0.43 mg/g dry weight in the transgenic rice plants	Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O	Panax ginseng	-	dammarenediol II	-	r

Organism

Organism	UniProt	Comment	Textmining
no activity in Oryza sativa	-	-	-
Panax ginseng	Q08IT1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O	-	Panax ginseng	dammarenediol II	-	r

Synonyms

Synonyms	Comment	Organism
dammarenediol-II synthase	-	Panax ginseng
DDS	-	Panax ginseng

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Release 2023.1 (January 2023)