Cloned (Comment) | Organism |
---|---|
gene DDS, recombinant expression in Pichia pastoris using the promoter of the AOX1 gene, coexpression with an ERG1 gene and an ERG7 gene | Panax ginseng |
Protein Variants | Comment | Organism |
---|---|---|
additional information | metabolic engineering of Pichia pastoris strain GS115 for the high yield production of dammarenediol-II, based on the native triterpene synthetic pathway, a dammarenediol-II synthetic pathway is established in Pichia pastoris by introducing a dammarenediol-II synthase gene (PgDDS) from Panax ginseng. To increase the supply of 2,3-oxidosqualene, the ERG1 gene, which is responsible for 2,3-oxidosqualene synthesis, is coexpressed. And to reduce competition for 2,3-oxidosqualene from ergosterol biosynthesis without affecting the normal growth of Pichia pastoris, the ERG7gene, which is responsible for conversion of 2,3-oxidosqualene to lanosterol, is downregulated by replacing its native promoter with a thiamine-repressible promoter, using a marker-recycling and gene-targeting Cre- lox71/66 system, developed for Pichia pastoris. Method evaluation and optimization, overview | Panax ginseng |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | Panax ginseng | - |
dammarenediol II | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panax ginseng | Q08IT1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O | - |
Panax ginseng | dammarenediol II | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dammarenediol-II synthase | - |
Panax ginseng |
DDS | - |
Panax ginseng |
PgDDS | - |
Panax ginseng |
PNA | - |
Panax ginseng |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Panax ginseng |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Panax ginseng |
General Information | Comment | Organism |
---|---|---|
physiological function | dammarenediol-II synthase gene (PgDDS) from Panax ginseng is responsible for the cyclization of 2,3-oxidosqualene to dammarenediol-II, the nucleus of dammarane-type ginsenosides, which are a group of active triterpenoids exhibiting various pharmacological activities | Panax ginseng |