BRENDA - Enzyme Database
show all sequences of 4.2.1.125

Dammarenediol-II production confers TMV tolerance in transgenic tobacco expressing Panax ginseng dammarenediol-II synthase

Lee, M.H.; Han, J.Y.; Kim, H.J.; Kim, Y.S.; Huh, G.H.; Choi, Y.E.; Plant Cell Physiol. 53, 173-182 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene PgDDS, functional expression in Nicotiana tabacum cv. Xanthi leaves using Agrobacterium strain GV3101, the heterologous expression confers resistance to Tobacco mosaic virus. Expression profile of PgDDS trangenic tobacco plants, overview. Production of dammarenediol-II in transgenic tobacco stimulates the expression of tobacco pathogenesis-related genes (PR1 and PR2) under both virus-untreated and -treated conditions
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
Panax ginseng
dammarenediol-II is a ginsenoside precursor
dammarenediol II
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Panax ginseng
Q08IT1
gene PgDDS
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
730586
Panax ginseng
dammarenediol II
GC-MS product analysis
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
dammarenediol-II is a ginsenoside precursor
730586
Panax ginseng
dammarenediol II
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
dammarenediol-II synthase
-
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PgDDS, functional expression in Nicotiana tabacum cv. Xanthi leaves using Agrobacterium strain GV3101, the heterologous expression confers resistance to Tobacco mosaic virus. Expression profile of PgDDS trangenic tobacco plants, overview. Production of dammarenediol-II in transgenic tobacco stimulates the expression of tobacco pathogenesis-related genes (PR1 and PR2) under both virus-untreated and -treated conditions
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
Panax ginseng
dammarenediol-II is a ginsenoside precursor
dammarenediol II
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
730586
Panax ginseng
dammarenediol II
GC-MS product analysis
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
dammarenediol-II is a ginsenoside precursor
730586
Panax ginseng
dammarenediol II
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first step in the synthesis of dammarane-type ginsenoside, the cyclization of 2,3-oxidosqualene to dammarenediol-II, overview
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first step in the synthesis of dammarane-type ginsenoside, the cyclization of 2,3-oxidosqualene to dammarenediol-II, overview
Panax ginseng
Other publictions for EC 4.2.1.125
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748118
Liu
-
Molecular simulation and cata ...
Panax ginseng
J. Beijing Inst. Technol.
26
563-570
2017
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747390
Li
Heterologous biosynthesis of ...
Panax ginseng
Biotechnol. Lett.
38
603-609
2016
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748874
Cheng
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SNPs of dammarenediol synthas ...
Panax ginseng
Phytochem. Lett.
17
194-200
2016
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2
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748268
Liu
Metabolic engineering of Pich ...
Panax ginseng
J. Biotechnol.
216
47-55
2015
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1
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1
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4
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1
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749016
Huang
Production of dammarane-type ...
no activity in Oryza sativa, Panax ginseng
Plant Sci.
239
106-114
2015
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1
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1
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6
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729785
Wang
The isolation and characteriza ...
Panax quinquefolius
Funct. Integr. Genomics
14
545-557
2014
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730594
Han
Production of dammarenediol-II ...
Panax ginseng
Plant Cell Rep.
33
225-233
2014
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729358
Hu
Molecular cloning, expression, ...
Panax ginseng
BioMed Res. Int.
2013
285740
2013
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730302
Liang
Heterologous expression of dam ...
Panax ginseng
Lett. Appl. Microbiol.
55
323-329
2012
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730586
Lee
Dammarenediol-II production co ...
Panax ginseng
Plant Cell Physiol.
53
173-182
2012
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710269
Kim
Upregulation of phytosterol an ...
Centella asiatica
Plant Cell Rep.
29
403-411
2010
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710295
Kim
Characterization of a dammaren ...
Centella asiatica
Plant Physiol. Biochem.
47
998-1002
2009
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683467
Tansakul
Dammarenediol-II synthase, the ...
Panax ginseng
FEBS Lett.
580
5143-5149
2006
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683979
Han
Expression and RNA interferenc ...
Panax ginseng
Plant Cell Physiol.
47
1653-1662
2006
1
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683213
Kushiro
In vitro conversion of 2,3-oxi ...
Panax ginseng
Biol. Pharm. Bull.
20
292-294
1997
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