Application | Comment | Organism |
---|---|---|
synthesis | the enzyme participates in psilocybin formation from 4-hydroxyindole and L-serine, which are less cost-intensive substrates, compared to the previous method. The pharmaceutical interest in this psychotropic natural product as a future medication to treat depression and anxiety is strongly reemerging. Enzymatic production of 7-phosphoryloxytryptamine (isonorbaeocystin), a non-natural congener of the Psilocybe alkaloid norbaeocystin (4-phosphoryloxytryptamine), and of serotonin (5-hydroxytryptamine) by means of the same in vitro approach | Psilocybe cubensis |
Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged TrpB in Escherichia coli strain KRX | Psilocybe cubensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions, in vitro reconstituted indole alkaloid synthesis pathway including enzyme PsiD, PsiK and PsiM, and ATP and S-adenosyl-L-methionine, method, overview. Assays run only with TrpB and PsiD result in identical chromatograms | Psilocybe cubensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Psilocybe cubensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + indole | Psilocybe cubensis | - |
L-tryptophan + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psilocybe cubensis | A0A2U8JPY3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + 4-hydroxyindole | - |
Psilocybe cubensis | 4-hydroxy-L-tryptophan + H2O | - |
? | |
L-serine + 5-hydroxyindole | - |
Psilocybe cubensis | 5-hydroxy-L-tryptophan + H2O | - |
? | |
L-serine + 7-hydroxyindole | - |
Psilocybe cubensis | 7-hydroxy-L-tryptophan + H2O | - |
? | |
L-serine + indole | - |
Psilocybe cubensis | L-tryptophan + H2O | - |
? | |
L-serine + indole | L-serine is the preferred substrate | Psilocybe cubensis | L-tryptophan + H2O | - |
? | |
additional information | enzymatic production of psilocybin formation from 4-hydroxyindole and L-serine and of 7-phosphoryloxytryptamine (isonorbaeocystin), a non-natural congener of the Psilocybe alkaloid norbaeocystin (4-phosphoryloxytryptamine), and of serotonin (5-hydroxytryptamine) by means of the same in vitro approach, overview | Psilocybe cubensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75500, about, TrpB monomer, sequence calculation | Psilocybe cubensis |
Synonyms | Comment | Organism |
---|---|---|
TrpBbeta | - |
Psilocybe cubensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Psilocybe cubensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Psilocybe cubensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Psilocybe cubensis | TrpB monomer, sequence calculation | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is part of the tryptophan synthase complex. Indole formation is catalyzed by the alpha-subunit (TrpBalpha), L-tryptophan production is catalyzed by the beta-subunit (TrpBbeta) | Psilocybe cubensis |