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Literature summary for 4.2.1.122 extracted from
- The tryptophan synthase beta-subunit paralogs TrpB1 and TrpB2 in Thermococcus kodakarensis are both involved in tryptophan biosynthesis and indole salvage (2014), FEBS J., 281, 3113-3125.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene trpB1, phylogenetic tree, expression in Escherichia coli strain BL21, transcript levels of trpB1 in the host, DELTAtrpB1 strains by quantitative RT-PCR expression analysis | Thermococcus kodakarensis |
| gene trpB2, phylogenetic tree, expression in Escherichia coli strain BL21, transcript levels of trpB2 in the host, DELTAtrpB2 strains by quantitative RT-PCR expression analysis | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state Michaelis-Menten kinetics, overview | Thermococcus kodakarensis | |
| 0.0076 | - |
indole | pH 7.5, 85°C, recombinant TrpB2, with TrpA | Thermococcus kodakarensis |  |
| 0.0081 | - |
indole | pH 7.5, 85°C, recombinant TrpB2, without TrpA | Thermococcus kodakarensis | |
| 0.035 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 with TrpA | Thermococcus kodakarensis | |
| 0.063 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 | Thermococcus kodakarensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + indole | Thermococcus kodakarensis | - |
L-tryptophan + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q5JDJ1 | TrpB2; gene trpB2, located outside the trpCDEGFB1A operon | - |
| Thermococcus kodakarensis | Q9YGB0 | TrpB1; gene trpB1, located adjacently to gene trpA in the trpCDEGFB1A operon | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + indole | - |
Thermococcus kodakarensis | L-tryptophan + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | Trp synthase is a heterotetramer composed of TrpA and TrpB, TrpA and TrpB1 assemble to form an alphabetabetaalpha heterotetramer | Thermococcus kodakarensis |
| More | complex formation of TrpB1 and TrpB2 with TrpA is analyzed by gel filtration | Thermococcus kodakarensis |
| More | complex formation of TrpB1 and TrpB2 with TrpA is analyzed by gel filtration, TrpA and TrpB2 do not form a complex | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trp synthase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
assay at | Thermococcus kodakarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
indole | pH 7.5, 85°C, recombinant TrpB2, with or without TrpA | Thermococcus kodakarensis | |
| 1.04 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 | Thermococcus kodakarensis | |
| 3.46 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 with TrpA | Thermococcus kodakarensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermococcus kodakarensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Thermococcus kodakarensis | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermococcus kodakarensis | transcript level of trpB1 (within the trp operon) increases by 110fold in Trp(-) medium compared to those in Trp(+) medium | up |
| Thermococcus kodakarensis | transcript level of trpB2 increases by 45fold in Trp(-) medium compared to those in Trp(+) medium | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the double-deletion mutant (DELTAtrpB1DELTAtrpB2) displays Trp auxotrophy, whereas individual single mutants (DELTAtrpB1 and DELTAtrpB2 strains) does not | Thermococcus kodakarensis |
| metabolism | the last two steps of L-tryptophan (Trp) biosynthesis are catalyzed by Trp synthase, a heterotetramer composed of TrpA and TrpB. TrpB catalyzes the condensation of indole, synthesized by TrpA, and serine to Trp. TrpB2 catalyzes the TrpB reaction but does not interact with TrpA as in the case of TrpB1. TrpB1 and TrpB2 both contribute to Trp biosynthesis in Thermococcus kodakarensis and can utilize free indole, and indole salvage does not necessarily rely on TrpB2 to a greater extent | Thermococcus kodakarensis |
| physiological function | trpB2 contributes in Trp biosynthesis | Thermococcus kodakarensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 | Thermococcus kodakarensis | |
| 48 | - |
indole | pH 7.5, 85°C, recombinant TrpB2, without TrpA | Thermococcus kodakarensis | |
| 51 | - |
indole | pH 7.5, 85°C, recombinant TrpB2, with TrpA | Thermococcus kodakarensis | |
| 99 | - |
indole | pH 7.5, 85°C, recombinant TrpB1 with TrpA | Thermococcus kodakarensis | |
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