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Literature summary for 4.2.1.120 extracted from

  • Scherf, U.; Buckel, W.
    Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase from Clostridium aminobutyricum (1993), Eur. J. Biochem., 215, 421-429.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme does not require ATP, MgCl2, and Ti(III)citrate Clostridium aminobutyricum

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is not inactivated by 5 mM 4-nitrophenol, 5 mM chloramphenicol, and 5 mM hydroxylamine Clostridium aminobutyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 4-hydroxybutanoyl-CoA pH 9.0, 25°C Clostridium aminobutyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 16 mol per mol of enzyme, plus 14 mol sulfur per mol of enzyme Clostridium aminobutyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Clostridium aminobutyricum
232000
-
 gel filtration Clostridium aminobutyricum

Organism

Organism UniProt Comment Textmining
Clostridium aminobutyricum
-
-
-

Oxidation Stability

Oxidation Stability Organism
exposure to air results in initial activation followed by irreversible inactivation Clostridium aminobutyricum

Purification (Commentary)

Purification (Comment) Organism
using anaerobic conditions Clostridium aminobutyricum

Reaction

Reaction Comment Organism Reaction ID
4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O cleavage is achieved by a FAD-dependent oxidation of 4-hydroxybutanoyl-CoA to 4-hydroxycrotonyl-CoA. In a second step, the hydroxyl group is substituted by a hydride derived from the now reduced FAD in an SN2' reaction leading to vinylacetyl-CoA. Isomerization yields crotonyl-CoA Clostridium aminobutyricum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.5
-
 pH 9.0, 25°C Clostridium aminobutyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybutanoyl-CoA
-
 Clostridium aminobutyricum vinylacetyl-CoA + H2O
-
 r
additional information no substrate: cyclopropylcarboxyl-CoA Clostridium aminobutyricum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000, SDS-PAGE Clostridium aminobutyricum

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of enzyme Clostridium aminobutyricum