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Literature summary for 4.2.1.120 extracted from

  • Scherf, U.; Söhling, B.; Gottschalk, G.; Linder, D.; Buckel, W.
    Succinate-ethanol fermentation in Clostridium kluyveri: purification, characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase (1994), Arch. Microbiol., 161, 239-245.
    View publication on PubMed
Search for more literature for 4.2.1.120

Inhibitors

Inhibitors Comment Organism Structure
Dithionite inactivation, oxidation with potassium hexacyanoferrate(III) results in up to 84% reactivation Clostridium kluyveri

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble high enzymic activity in anaerobically grown cell extract grown on succinate plus ethanol Clostridium kluyveri
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 13.6 mol iron per mol of enzyme Clostridium kluyveri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 4 * 59000, SDS-PAGE Clostridium kluyveri
237000
-
 gel filtration Clostridium kluyveri

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri
-
-
-

Oxidation Stability

Oxidation Stability Organism
exposure to air leads to irreversible inactivation Clostridium kluyveri

Purification (Commentary)

Purification (Comment) Organism
purification under strictly anaerobic conditions Clostridium kluyveri

Renatured (Commentary)

Renatured (Comment) Organism
enzyme is inactivated by dithionite, oxidation with potassium hexacyanoferrate(III) results in up to 84% reactivation Clostridium kluyveri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.38
-
-
 Clostridium kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybutanoyl-CoA
-
 Clostridium kluyveri but-3-enoyl-CoA + H2O
-
 ?
additional information enzyme has an intrinsic vinylacetyl-CoA isomerase activity Clostridium kluyveri ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, SDS-PAGE Clostridium kluyveri

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol FAD per mol of enzyme Clostridium kluyveri