Cloned (Comment) | Organism |
---|---|
gene CG3415, Drosophila melanogaster MFE-2 complements a Saccharomyces cerevisiae MFE-2 deletion strain, functional expression of His-tagged MFE-2 in Escherichia coli strain BL21(DE3) pLysS | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged MFE-2, 5 mg/ml protein in 0.1 Msodium phosphate, pH 7.2, and 0.2 M NaF, sitting and hanging drop vapour diffusion methods are used at 21°C, mixing of equal volumes of protein and reservoir solutions, the latter contains 100 mM Tris-HCl, pH 8.0, 1.0 M NaCl, 20% w/v PEG 5000 MME and 5 mM NAD+, X-ray diffraction structure determination and analysis at 2.15 A resolution | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics using the DmDH and DmH2 domains of DmMFE-2 as separate monofunctional enzymes at a 1:1 ratio, overview | Drosophila melanogaster | |
0.00114 | - |
(2E)-decenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
0.0667 | - |
(2E)-hexenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
0.0853 | - |
(2E)-butenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Drosophila melanogaster | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64100 | - |
2 * 64100, MFE-2, SDS-PAGE | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-enoyl-CoA + H2O | Drosophila melanogaster | - |
(3R)-hydroxyacyl-CoA | - |
? | |
additional information | Drosophila melanogaster | the bifunctional peroxisomal multifunctional enzyme type 2 exhibits dehydrogenase and hydratase activity from separate entities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
gene CG3415 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MFE-2 from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography, removal of the His tag | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-butenoyl-CoA + H2O | - |
Drosophila melanogaster | (3R)-hydroxybutanoyl-CoA | - |
? | |
(2E)-decenoyl-CoA + H2O | - |
Drosophila melanogaster | (3R)-3-hydroxydecanoyl-CoA | - |
? | |
(2E)-enoyl-CoA + H2O | - |
Drosophila melanogaster | (3R)-hydroxyacyl-CoA | - |
? | |
(2E)-hexenoyl-CoA + H2O | - |
Drosophila melanogaster | (3R)-3-hydroxyhexanoyl-CoA | - |
? | |
additional information | the bifunctional peroxisomal multifunctional enzyme type 2 exhibits dehydrogenase and hydratase activity from separate entities | Drosophila melanogaster | ? | - |
? | |
additional information | MFE-2 structure-function studies, overview | Drosophila melanogaster | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 64100, MFE-2, SDS-PAGE | Drosophila melanogaster |
More | necessity of dimerization, domain organization, MFE-2 structure-function studies, overview | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
2E-enoyl-CoA hydratase 2 | - |
Drosophila melanogaster |
MFE-2 | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
(2E)-butenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
210 | - |
(2E)-hexenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
1100 | - |
(2E)-decenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000446 | - |
(2E)-butenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
0.0032 | - |
(2E)-hexenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster | |
0.97 | - |
(2E)-decenoyl-CoA | pH 7.5, 22°C, recombinant full-length enzyme | Drosophila melanogaster |