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Literature summary for 4.2.1.119 extracted from
- Alteration of chain length substrate specificity of Aeromonas caviae R-enantiomer-specific enoyl-coenzyme A hydratase through site-directed mutagenesis (2003), Appl. Environ. Microbiol., 69, 4830-4836.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Aeromonas caviae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L654G/V130G | decreased specific activity for crotonyl-CoA | Aeromonas caviae |
| L65A | enzyme activity similar to wild-type enzyme | Aeromonas caviae |
| L65A/V130G | decreased specific activity for crotonyl-CoA | Aeromonas caviae |
| L65G | enzyme activity similar to wild-type enzyme | Aeromonas caviae |
| L65I | specific activity with crotonyl-CoA similar to wild-type enzyme | Aeromonas caviae |
| L65V | specific activity with crotonyl-CoA similar to wild-type enzyme | Aeromonas caviae |
| S62A | decreased specific activity for crotonyl-CoA | Aeromonas caviae |
| V130A | specific activity with crotonyl-CoA similar to wild-type enzyme | Aeromonas caviae |
| V130G | enzyme activity similar to wild-type enzyme, lower structural stability than wild-type enzyme | Aeromonas caviae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
dodec-2-enoyl-CoA | pH 8, 30°C, V130G mutant | Aeromonas caviae |  |
| 0.013 | - |
dec-2-enoyl-CoA | pH 8, 30°C, V130G mutant | Aeromonas caviae | |
| 0.018 | - |
hexenoyl-CoA | pH 8, 30°C, L65A mutant | Aeromonas caviae | |
| 0.021 | - |
(2E)-oct-2-enoyl-CoA | pH 8, 30°C, L65A mutant | Aeromonas caviae | |
| 0.024 | - |
crotonyl-CoA | pH 8, 30°C, wild-type | Aeromonas caviae | |
| 0.027 | - |
dec-2-enoyl-CoA | pH 8, 30°C, L65A mutant | Aeromonas caviae | |
| 0.033 | - |
crotonyl-CoA | pH 8, 30°C, L65A mutant | Aeromonas caviae | |
| 0.034 | - |
dodec-2-enoyl-CoA | pH 8, 30°C, L65A mutant | Aeromonas caviae | |
| 0.04 | - |
hexenoyl-CoA | pH 8, 30°C, wild-type | Aeromonas caviae | |
| 0.042 | - |
dec-2-enoyl-CoA | pH 8, 30°C, wild-type | Aeromonas caviae | |
| 0.042 | - |
(2E)-oct-2-enoyl-CoA | pH 8, 30°C, wild-type | Aeromonas caviae | |
| 0.043 | - |
dodec-2-enoyl-CoA | pH 8, 30°C, wild-type | Aeromonas caviae | |
| 0.076 | - |
(2E)-oct-2-enoyl-CoA | pH 8, 30°C, V130G mutant | Aeromonas caviae | |
| 0.102 | - |
hexenoyl-CoA | pH 8, 30°C, V130G mutant | Aeromonas caviae | |
| 0.154 | - |
crotonyl-CoA | pH 8, 30°C, V130G mutant | Aeromonas caviae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas caviae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
L654G/V130G mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 0.08 | - |
L65A/V130G mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 0.55 | - |
S62A mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 0.86 | - |
wild-type with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 1.98 | - |
L65G mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 2.25 | - |
L65I mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 6.59 | - |
V130A mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 7.92 | - |
L65V mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 15.8 | - |
L65G mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 21.2 | - |
V130G mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 67 | - |
S62A mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 68.5 | - |
V130G mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 69.8 | - |
L65A mutant with octenoyl-CoA as substrate, cell extract | Aeromonas caviae |
| 883 | - |
V130G mutant with crotonyl-CoA as substrate, purified enzyme | Aeromonas caviae |
| 1256 | - |
L65A mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 1288 | - |
V130A mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 1538 | - |
L65V mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 1594 | - |
wild-type with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
| 1880 | - |
L65I mutant with crotonyl-CoA as substrate, cell extract | Aeromonas caviae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxybutanoyl-CoA | - |
? | |
| dec-2-enoyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxydecanoyl-CoA | - |
? | |
| dodec-2-enoyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxydodecanoyl-CoA | - |
? | |
| hexenoyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxyhexanoyl-CoA | - |
? | |
| oct-2-enoyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxyoctanoyl-CoA | - |
? | |
| trans-2-octenoyl-CoA + H2O | - |
Aeromonas caviae | 3-hydroxyoctanoyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhaJAc | - |
Aeromonas caviae |
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