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Literature summary for 4.2.1.119 extracted from
- Enzymes converting D-3-hydroxyacyl-CoA to trans-2-enoyl-CoA. Microsomal and peroxisomal isoenzymes in rat liver (1993), J. Biol. Chem., 268, 21578-21585.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | enzyme different from peroxisomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart | Rattus norvegicus | - |
- |
| peroxisome | enzyme different from microsomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart | Rattus norvegicus | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31500 | - |
2 * 31500, microsomal isoform, SDS-PAGE | Rattus norvegicus |
| 33500 | - |
2 * 33500, peroxisomal isoform, SDS-PAGE | Rattus norvegicus |
| 60000 | - |
microsomal isoform, gel filtration | Rattus norvegicus |
| 62000 | - |
peroxisomal isoform, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 0.2% yield,the microsomal isoform elutes at 0.4-0.7 MKCl, at low salt concentrations, whereas the peroxisomal isoform elutes at high salt concentrations | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.5 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxydecanoyl-CoA | - |
Rattus norvegicus | trans-2-decenoyl-CoA + H2O | - |
r |  |
| additional information | the (S)-3-hydroxy-CoA is not dehydrated | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 31500, microsomal isoform, SDS-PAGE | Rattus norvegicus |
| homodimer | 2 * 33500, peroxisomal isoform, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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