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Literature summary for 4.2.1.117 extracted from
- The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo (2004), J. Bacteriol., 186, 454-462.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Shewanella oneidensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete loss of activity | Shewanella oneidensis |  |
| ferricyanide | complete loss of activity | Shewanella oneidensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | enzyme requires an intact Fe/S cluster for activity | Shewanella oneidensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
x * 94000, SDS-PAGE and calculated | Shewanella oneidensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shewanella oneidensis | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| in presence of air, activity is lost over time | Shewanella oneidensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
pH 8.0, 37°C, substrate citrate | Shewanella oneidensis |
| 5 | - |
pH 8.0, 37°C, substrate cis-aconitate | Shewanella oneidensis |
| 7.8 | - |
pH 8.0, 37°C, substrate 2-methylcitrate | Shewanella oneidensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylcitrate | - |
Shewanella oneidensis | 2-methyl-cis-aconitate + H2O | - |
? | |
| cis-aconitate + H2O | - |
Shewanella oneidensis | citrate | - |
? | |
| citrate | - |
Shewanella oneidensis | cis-aconitate + H2O | - |
r | |
| additional information | no substrate: 2-methylisocitrate, isocitrate. Enzyme only catalyzes the first half of the aconitase-like reaction | Shewanella oneidensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 94000, SDS-PAGE and calculated | Shewanella oneidensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcnD | - |
Shewanella oneidensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4Fe-4S-center | enzyme requires an intact Fe/S cluster for activity | Shewanella oneidensis | |
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Release 2023.1 (January 2023)
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