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Literature summary for 4.2.1.115 extracted from
- Topological and functional characterization of WbpM, an inner membrane UDP-GlcNAc C6 dehydratase essential for lipopolysaccharide biosynthesis in Pseudomonas aeruginosa (2001), Mol. Microbiol., 41, 1295-1310.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type N-terminally His-tagged and C-terminally His-tagged WbpMs, and mutant truncated WbpM in membranes of Escherichia coli, reaction yields are lower with C-terminally His-tagged than with N-terminally His-tagged recombinant enzyme | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a soluble truncated form of WbpM, His-S262 | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.77 | - |
UDP-N-acetylglucosamine | pH 10.0, 30°C, recombinant soluble truncated WbpM mutant His-S262 | Pseudomonas aeruginosa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inner membrane | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 63000, recombinant N-terminally His-tagged WbpM, SDS-PAGE, x * 64000, recombinant C-terminally His-tagged WbpM, SDS-PAGE, x * 60000, recombinant His-tagged truncated WbpM, SDS-PAGE | Pseudomonas aeruginosa |
| 63000 | - |
x * 63000, recombinant N-terminally His-tagged WbpM, SDS-PAGE, x * 64000, recombinant C-terminally His-tagged WbpM, SDS-PAGE, x * 60000, recombinant His-tagged truncated WbpM, SDS-PAGE | Pseudomonas aeruginosa |
| 64000 | - |
x * 63000, recombinant N-terminally His-tagged WbpM, SDS-PAGE, x * 64000, recombinant C-terminally His-tagged WbpM, SDS-PAGE, x * 60000, recombinant His-tagged truncated WbpM, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas aeruginosa | WbpM is essential for the biosynthesis of B-band lipopolysaccharide in many serotypes of Pseudomonas aeruginosa | ? | - |
? | |
| UDP-N-acetylglucosamine | Pseudomonas aeruginosa | WbpM is specific for UDP-GlcNAc | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | P72145 | gene wbpM | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His-tagged and C-terminally His-tagged WbpMs from Escherichia coli by selective solubilization from the inner membrane by lauryl sarcosine and nickel chelation chromatography | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | reaction mechanism of WbpM | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | WbpM is essential for the biosynthesis of B-band lipopolysaccharide in many serotypes of Pseudomonas aeruginosa | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | no activity with UDP-Glc, UDP-GalNAc or UDP-Gal, and with substrates of other known C6 dehydratases such as GDP-mannose, dTDP-Glc and CDP-Glc. Structure-function analysis, although the membrane domains do not have any catalytic activity, they are important for the polymerization of high-molecular weight B-band lipopolysaccharide, overview | Pseudomonas aeruginosa | ? | - |
? | |
| UDP-N-acetylglucosamine | WbpM is specific for UDP-GlcNAc | Pseudomonas aeruginosa | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? | |
| UDP-N-acetylglucosamine | WbpM is specific for UDP-GlcNAc. Although WbpM possesses an altered catalytic triad composed of SMK as opposed to SYK commonly found in other dehydratases, its catalysis is very efficient | Pseudomonas aeruginosa | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, recombinant N-terminally His-tagged WbpM, SDS-PAGE, x * 64000, recombinant C-terminally His-tagged WbpM, SDS-PAGE, x * 60000, recombinant His-tagged truncated WbpM, SDS-PAGE | Pseudomonas aeruginosa |
| More | WbpM is anchored to the inner membrane via four N-terminal transmembrane domains, whereas the C-terminal catalytic domain resided in the cytoplasm, topological model of WbpM, overview | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-GlcNAc C6 dehydratase | - |
Pseudomonas aeruginosa |
| WbpM | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
UDP-N-acetylglucosamine | pH 10.0, 30°C, recombinant soluble truncated WbpM mutant His-S262 | Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
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