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Literature summary for 4.2.1.115 extracted from

  • Creuzenet, C.; Urbanic, R.V.; Lam, J.S.
    Structure-function studies of two novel UDP-GlcNAc C6 dehydratases/C4 reductases. Variation from the SYK dogma (2002), J. Biol. Chem., 277, 26769-26778.
    View publication on PubMed
Search for more literature for 4.2.1.115

Protein Variants

Protein Variants Comment Organism
C103M site-directed mutagenesis, the mutant is inactive, dimerization is prevented but the secondary structure is not significantly affected Helicobacter pylori
C118M site-directed mutagenesis, not recombinantly expressable mutant Helicobacter pylori
D149K/K150A site-directed mutagenesis, the mutant is inactive Helicobacter pylori
D149K/K150D site-directed mutagenesis, the mutant is inactive Helicobacter pylori
D70A site-directed mutagenesis, the mutant is inactive Helicobacter pylori
D70N site-directed mutagenesis, not recombinantly expressable mutant Helicobacter pylori
G20A site-directed mutagenesis, the mutant is inactive Helicobacter pylori
H86A site-directed mutagenesis, insoluble protein, the mutant shows reduced activity compared to the wild-type enzyme Helicobacter pylori
H86F site-directed mutagenesis, insoluble protein, the mutant is inactive Helicobacter pylori
V266E site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Helicobacter pylori
Y141F site-directed mutagenesis, the mutant is inactive Helicobacter pylori
Y141M site-directed mutagenesis of a FlaA1 catalytic triad mutant, the mutant shows slightly reduced activity compared to the wild-type enzyme Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.151
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant V266E Helicobacter pylori
0.159
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, wild-type enzyme Helicobacter pylori
0.251
-
 UDP-N-acetylglucosamine pH 10.0, 37°C, mutant Y141M Helicobacter pylori
0.494
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant H86A Helicobacter pylori
0.565
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant Y141M Helicobacter pylori

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Helicobacter pylori
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetylglucosamine Helicobacter pylori
-
 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with UDP-Glc, UDP-Gal, or UDP-GalNAc or with substrates of other known C6 dehydratases, i.e. GDP-mannose or dTDP-glucose, structure-function analysis, overview Helicobacter pylori ?
-
 ?
UDP-N-acetylglucosamine
-
 Helicobacter pylori UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O
-
 ?
UDP-N-acetylglucosamine the enzyme catalyzes the stereospecific conversion of UDP-GlcNAc to Qui2NAc, i.e. 2-acetamido-2,6-dideoxy-D-glucose or N-acetylquinovosamine, via the formation of a 4-keto, 6-deoxy intermediate Helicobacter pylori UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O
-
 ?

Subunits

Subunits Comment Organism
More FlaA1 is a short soluble protein that exhibits a typical SYK catalytic triad, structure-function analysis, overview Helicobacter pylori

Synonyms

Synonyms Comment Organism
FlaA1
-
 Helicobacter pylori
UDP-GlcNAc C6 dehydratase
-
 Helicobacter pylori
UDP-GlcNAc C6 dehydratase/C4 reductase
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant Y141M Helicobacter pylori
0.065
-
 UDP-N-acetylglucosamine pH 10.0, 37°C, mutant Y141M Helicobacter pylori
0.077
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant H86A Helicobacter pylori
0.095
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, wild-type enzyme Helicobacter pylori
0.16
-
 UDP-N-acetylglucosamine pH 7.0, 37°C, mutant V266E Helicobacter pylori