Any feedback?
Literature summary for 4.2.1.112 extracted from
- Acetylene hydratase of Pelobacter acetylenicus. Molecular and spectroscopic properties of the tungsten iron-sulfur enzyme (1999), Eur. J. Biochem., 264, 176-182.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is inducible by acetylene feeding | Syntrophotalea acetylenica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme activity depends on the redox status, overview | Syntrophotalea acetylenica |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | dependent on, the enzyme is a tungsten/iron-sulfur protein with [3Fe-4S], 2.0 gAV, low potential [4Fe-4S] cluster which is highly sensitive to oxidation, 4.8 mol of iron per mol of enzyme, 3.9 mol of acid-labile sulfur per mol of enzyme | Syntrophotalea acetylenica |  |
| Molybdenum | 1.3 mol of molybdopterin-guanine dinucleotide per mol of enzyme | Syntrophotalea acetylenica | |
| Tungsten | dependent on, the enzyme is a tungsten/iron-sulfur protein, 0.5 mol of tungsten per mol of enzyme | Syntrophotalea acetylenica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
1 * 73000, SDS-PAGE | Syntrophotalea acetylenica |
| 83550 | - |
MALDI mass spectrometry | Syntrophotalea acetylenica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene + H2O | Syntrophotalea acetylenica | - |
acetaldehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Syntrophotalea acetylenica | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the enzyme is oxygen-sensitive | Syntrophotalea acetylenica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 7.6fold by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration to homogeneity | Syntrophotalea acetylenica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the organism is grown in carbonate-buffered, sulfide-reduced, and tungstate-supplemented fresh water medium, optimally at pH 6.8-7.0 | Syntrophotalea acetylenica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13 | - |
crude cell extract of cells grown in presence of acetylene | Syntrophotalea acetylenica |
| 26.5 | - |
purified native enzyme | Syntrophotalea acetylenica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylene + H2O | - |
Syntrophotalea acetylenica | acetaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 73000, SDS-PAGE | Syntrophotalea acetylenica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Syntrophotalea acetylenica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Syntrophotalea acetylenica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| molybdopterin guanine dinucleotide | 1.3 mol of cofactor per mol of enzyme | Syntrophotalea acetylenica | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
