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Literature summary for 4.2.1.111 extracted from

  • Yu, S.; Fiskesund, R.
    The anhydrofructose pathway and its possible role in stress response and signaling (2006), Biochim. Biophys. Acta, 1760, 1314-1322.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 50 mM, 84% inhibition Anthracobia melaloma

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity Anthracobia melaloma
MgCl2 requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity Anthracobia melaloma
NaCl 0.1 mM, around 2fold increase in activity Anthracobia melaloma

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
2 * 98000, SDS-PAGE Anthracobia melaloma
230000
-
gel filtration Anthracobia melaloma

Organism

Organism UniProt Comment Textmining
Anthracobia melaloma
-
-
-
Plicaria carbonaria
-
-
-
Plicaria leiocarpa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose i.e. 1,5-anhydro-D-arabino-hex-2-ulose Anthracobia melaloma 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O i.e.ascopyrone M ir
1,5-anhydro-D-fructose i.e. 1,5-anhydro-D-arabino-hex-2-ulose Plicaria carbonaria 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O i.e.ascopyrone M ir
1,5-anhydro-D-fructose i.e. 1,5-anhydro-D-arabino-hex-2-ulose Plicaria leiocarpa 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O i.e.ascopyrone M ir
additional information the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone Anthracobia melaloma ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 98000, SDS-PAGE Anthracobia melaloma

Synonyms

Synonyms Comment Organism
AF dehydratase
-
Anthracobia melaloma
AF dehydratase
-
Plicaria carbonaria
AF dehydratase
-
Plicaria leiocarpa
AFDH
-
Anthracobia melaloma
AFDH
-
Plicaria carbonaria
AFDH
-
Plicaria leiocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
optimal pH-range is 5.9-7.0 Anthracobia melaloma

pH Range

pH Minimum pH Maximum Comment Organism
5.9 7 optimal pH-range Anthracobia melaloma