General Stability | Organism |
---|---|
Microthecin is found to be most stable in de-ionized water at a pH around 7.0 and stable in freeze-dried form. Under acidic conditions microthecin is degraded to 2-furyl-2-hydroxymethylketone (FHMK). | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-fructose | substrate inhibition to AUDH at a concentration up to 10% (w/v) | Phanerodontia chrysosporium | |
microthecin | product inhibition to AUDH at a concentration up to 10% (w/v) | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
glucosone | - |
Phanerodontia chrysosporium | |
5.4 | - |
1,5-Anhydro-D-fructose | - |
Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosone | Phanerodontia chrysosporium | 2-keto glucose | cortalcerone + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the best solvent for AUDH-catalysed microthecin reaction is de-ionized water | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | P84193 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose | - |
Phanerodontia chrysosporium | 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O | microthecin + H2O | ? | |
glucosone | 2-keto glucose | Phanerodontia chrysosporium | cortalcerone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldos-2-ulose dehydratase | bifunctional enzyme | Phanerodontia chrysosporium |
AUDH | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
at temperatures higher than 24°C there is increased degradation of microthecin | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the reaction medium acidifies by itself due to unknown events related to the AUDH catalysed reaction | Phanerodontia chrysosporium |
5 | - |
at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation | Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation | Phanerodontia chrysosporium |