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Literature summary for 4.2.1.110 extracted from

  • Yu, S.; Andreassen, M.; Lundt, I.
    Enzymatic production of microthecin by aldos-2-ulose dehydratase from 1,5-anhydro-D-fructose and stability studies of microthecin (2008), Biocatal. Biotransform., 26, 169-176.
No PubMed abstract available

General Stability

General Stability Organism
Microthecin is found to be most stable in de-ionized water at a pH around 7.0 and stable in freeze-dried form. Under acidic conditions microthecin is degraded to 2’-furyl-2-hydroxymethylketone (FHMK). Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
1,5-Anhydro-D-fructose substrate inhibition to AUDH at a concentration up to 10% (w/v) Phanerodontia chrysosporium
microthecin product inhibition to AUDH at a concentration up to 10% (w/v) Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.9
-
glucosone
-
Phanerodontia chrysosporium
5.4
-
1,5-Anhydro-D-fructose
-
Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucosone Phanerodontia chrysosporium 2-keto glucose cortalcerone + H2O
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the best solvent for AUDH-catalysed microthecin reaction is de-ionized water Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium P84193
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose
-
Phanerodontia chrysosporium 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O microthecin + H2O ?
glucosone 2-keto glucose Phanerodontia chrysosporium cortalcerone + H2O
-
?

Synonyms

Synonyms Comment Organism
aldos-2-ulose dehydratase bifunctional enzyme Phanerodontia chrysosporium
AUDH
-
Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
at temperatures higher than 24°C there is increased degradation of microthecin Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
the reaction medium acidifies by itself due to unknown events related to the AUDH catalysed reaction Phanerodontia chrysosporium
5
-
at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
at a pH lower than 5.0, the reaction rate is slower and there is a tendency for increased formation of degradation products (FHMK), at a pH higher than 5.5, there is a tendency for more APT dihydrate formation Phanerodontia chrysosporium