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Literature summary for 4.2.1.110 extracted from

  • Yu, S.
    Enzymatic description of the anhydrofructose pathway of glycogen degradation II. Gene identification and characterization of the reactions catalyzed by aldos-2-ulose dehydratase that converts 1,5-anhydro-D-fructose to microthecin with ascopyrone M as the intermediate (2005), Biochim. Biophys. Acta, 1723, 63-73.
    View publication on PubMed
Search for more literature for 4.2.1.110

Inhibitors

Inhibitors Comment Organism Structure
1,5-anhydro-4-deoxy-D-glycero-hexo-2,3-diulose dihydrate 4 mM, 50% inhibition when 1,5-anhydro-D-fructose is used as the substrate at 5 mM and pH 5.8 Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97400
-
 x * 97400, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-anhydro-D-fructose Phanerodontia chrysosporium 1,5-anhydro-D-fructose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O (overall reaction), (1a) 1,5-anhydro-D-fructose = 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O, (1b) 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one. This enzyme catalyses two of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose. The other enzymes involved in this pathway are EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase), EC 4.2.2.13 (exo-(1,4)-alpha-D-glucan lyase) and EC 5.3.3.15 (ascopyrone tautomerase). This is a bifunctional enzyme that acts as both a lyase and as an isomerase. Differs from EC 4.2.1.111, which can carry out only reaction 1a 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O i.e. microthecin ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose 1,5-anhydro-D-fructose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O (overall reaction), (1a) 1,5-anhydro-D-fructose = 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O, (1b) 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one. This enzyme catalyses two of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose. The other enzymes involved in this pathway are EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase), EC 4.2.2.13 (exo-(1,4)-alpha-D-glucan lyase) and EC 5.3.3.15 (ascopyrone tautomerase). This is a bifunctional enzyme that acts as both a lyase and as an isomerase. Differs from EC 4.2.1.111, which can carry out only reaction 1a Phanerodontia chrysosporium 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O i.e. microthecin ?
1,5-anhydro-D-fructose formation of microthecin is irreversible. 1,5-anhydro-D-fructose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O (overall reaction), (1a) 1,5-anhydro-D-fructose = 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O, (1b) 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose = 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one. This enzyme catalyses two of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose. The other enzymes involved in this pathway are EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase), EC 4.2.2.13 (exo-(1,4)-alpha-D-glucan lyase) and EC 5.3.3.15 (ascopyrone tautomerase). This is a bifunctional enzyme that acts as both a lyase and as an isomerase. Differs from EC 4.2.1.111, which can carry out only reaction 1a Phanerodontia chrysosporium 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H2O i.e. microthecin ir
glucosone 20% of the activity with 1,5-anhydro-D-fructose Phanerodontia chrysosporium cortalcerone + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 97400, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
AUDH
-
 Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 formation of 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose Phanerodontia chrysosporium
6.8
-
 formation of microthecin Phanerodontia chrysosporium