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Literature summary for 4.2.1.11 extracted from

  • Kornblatt, M.J.; Richard Albert, J.; Mattie, S.; Zakaib, J.; Dayanandan, S.; Hanic-Joyce, P.J.; Joyce, P.B.
    The Saccharomyces cerevisiae enolase-related regions encode proteins that are active enolases (2013), Yeast, 30, 55-69.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibitory at higher concentrations Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03205
-
 2-phospho-D-glycerate isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
0.04482
-
 2-phospho-D-glycerate isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
0.05856
-
 2-phospho-D-glycerate isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enolase is activated by low concentrations of Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46672
-
 x * 46672, isoform Eno1p, calculated from amino acid sequence Saccharomyces cerevisiae
46673
-
 x * 46673, isoform Eno1p, electrospray ionization mass spectrometry Saccharomyces cerevisiae
47181
-
 x * 47181, isoform Err3p, calculated from amino acid sequence Saccharomyces cerevisiae
47184
-
 x * 47184, isoform Err3p, electrospray ionization mass spectrometry Saccharomyces cerevisiae
47196
-
 x * 47196, isoform Err2p, calculated from amino acid sequence Saccharomyces cerevisiae
47198
-
 x * 47198, isoform Err2p, electrospray ionization mass spectrometry Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate Saccharomyces cerevisiae BY4743
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4743
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate
-
 Saccharomyces cerevisiae BY4743 phosphoenolpyruvate + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 46672, isoform Eno1p, calculated from amino acid sequence Saccharomyces cerevisiae
? x * 46673, isoform Eno1p, electrospray ionization mass spectrometry Saccharomyces cerevisiae
? x * 47181, isoform Err3p, calculated from amino acid sequence Saccharomyces cerevisiae
? x * 47184, isoform Err3p, electrospray ionization mass spectrometry Saccharomyces cerevisiae
? x * 47196, isoform Err2p, calculated from amino acid sequence Saccharomyces cerevisiae
? x * 47198, isoform Err2p, electrospray ionization mass spectrometry Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Eno1p
-
 Saccharomyces cerevisiae
enolase
-
 Saccharomyces cerevisiae
Err2p
-
 Saccharomyces cerevisiae
Err3p
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55.5
-
 the melting temperature of isoform Eno1p, Err2, and Err3p are 55.5°C, 60.5°C, and 60°C, respectively Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.4
-
 2-phospho-D-glycerate isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
19.7
-
 2-phospho-D-glycerate isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
71.4
-
 2-phospho-D-glycerate isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.327
-
 Mg2+ isoform Err2p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
0.695
-
 Mg2+ isoform Err3p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae
2.1
-
 Mg2+ isoform Eno1p, in 50 mM imidazole, pH 7.1, 250 mM KCl, 1 mM Mg(OAc)2, 0.1 mM EDTA, temperature not specified in the publication Saccharomyces cerevisiae