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Literature summary for 4.2.1.11 extracted from
- The hyperthermophilic glycolytic enzyme enolase in the archaeon, Pyrococcus furiousus. Comparison with mesophilic enolases (1994), Arch. Biochem. Biophys., 313, 280-286.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | competitive | Pyrococcus furiosus |  |
| EDTA | 1 mM | Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
2-phospho-D-glycerate | pH 8, 70°C | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM | Pyrococcus furiosus | |
| Mn2+ | metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000 | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | Pyrococcus furiosus | the enzyme probably functions in sugar fermentation pathway | phosphoenolpyruvate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q9UWJ5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.8 | - |
pH 8, 70°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Pyrococcus furiosus | phosphoenolpyruvate + H2O | - |
? | |
| 2-phospho-D-glycerate | the enzyme probably functions in sugar fermentation pathway | Pyrococcus furiosus | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 46000 | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enolase | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
above | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 88 | - |
t1/2: 300 min | Pyrococcus furiosus |
| 100 | - |
40 min, 50% inactivation. The half-life declines by a factor of about three in the presence of 700 mM KCl | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Pyrococcus furiosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
citrate | pH 8, 70°C | Pyrococcus furiosus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme probably functions in sugar fermentation pathway | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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