Application | Comment | Organism |
---|---|---|
medicine | the recombinant enzyme protein is a useful antigen in vaccination against adult worms | Schistosoma japonicum |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli | Schistosoma japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, forward and reverse reaction, overview | Schistosoma japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Schistosoma japonicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant His-tagged enolase, SDS-PAGE | Schistosoma japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Schistosoma japonicum | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma japonicum | Q5DDV5 | Anhui isolate, Chinese mainland strain, propagated in BALB/c mice | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Schistosoma japonicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enolase gene is highly expressed at 18-28 days of the life cycle, RT-PCR expression analysis | Schistosoma japonicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.87 | - |
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C | Schistosoma japonicum |
35.81 | - |
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C | Schistosoma japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Schistosoma japonicum | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant His-tagged enolase, SDS-PAGE | Schistosoma japonicum |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Schistosoma japonicum |
SjENO | - |
Schistosoma japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Schistosoma japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Schistosoma japonicum |
General Information | Comment | Organism |
---|---|---|
metabolism | enolase is a key enzyme in the glycolytic pathway | Schistosoma japonicum |
additional information | the recombinant enolase is recognized by rabbit sera directed against an antigen preparation from adult worms. 24.28% reduction in the liver egg count and a reduction of 21.45% in the fecal egg count occur in BALB/c mice vaccinated with recombinant SjENO compared with control mice | Schistosoma japonicum |
physiological function | recombinant SjENO binds to human plasminogen as its receptor | Schistosoma japonicum |