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Literature summary for 4.2.1.11 extracted from

  • Yang, J.; Qiu, C.; Xia, Y.; Yao, L.; Fu, Z.; Yuan, C.; Feng, X.; Lin, J.
    Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage (2010), Parasitol. Res., 107, 667-677.
    View publication on PubMed

Application

Application Comment Organism
medicine the recombinant enzyme protein is a useful antigen in vaccination against adult worms Schistosoma japonicum

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli Schistosoma japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis, forward and reverse reaction, overview Schistosoma japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Schistosoma japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, recombinant His-tagged enolase, SDS-PAGE Schistosoma japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Schistosoma japonicum
-
phosphoenolpyruvate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum Q5DDV5 Anhui isolate, Chinese mainland strain, propagated in BALB/c mice
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography Schistosoma japonicum

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enolase gene is highly expressed at 18-28 days of the life cycle, RT-PCR expression analysis Schistosoma japonicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.87
-
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C Schistosoma japonicum
35.81
-
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C Schistosoma japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Schistosoma japonicum phosphoenolpyruvate + H2O
-
r

Subunits

Subunits Comment Organism
? x * 50000, recombinant His-tagged enolase, SDS-PAGE Schistosoma japonicum

Synonyms

Synonyms Comment Organism
enolase
-
Schistosoma japonicum
SjENO
-
Schistosoma japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Schistosoma japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Schistosoma japonicum

General Information

General Information Comment Organism
metabolism enolase is a key enzyme in the glycolytic pathway Schistosoma japonicum
additional information the recombinant enolase is recognized by rabbit sera directed against an antigen preparation from adult worms. 24.28% reduction in the liver egg count and a reduction of 21.45% in the fecal egg count occur in BALB/c mice vaccinated with recombinant SjENO compared with control mice Schistosoma japonicum
physiological function recombinant SjENO binds to human plasminogen as its receptor Schistosoma japonicum