Literature summary for 4.2.1.11 extracted from

Schulz, E.C.; Tietzel, M.; Tovy, A.; Ankri, S.; Ficner, R.
Structure analysis of Entamoeba histolytica enolase (2011), Acta Crystallogr. Sect. D, 67, 619-627.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GST-tagged ENO in Escherichia coli BL21 (DE3)	Entamoeba histolytica

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant EhENO with two 2-phospho-D-glycerate substrate molecules bound at the two active sites, the protein solution contains 7.5 mg/ml protein in 100 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM MgCl2, sitting-drop vapour-diffusion, 20°C, mixing of equal volumes of precipitant solution, contraining 100 mM Bis-Tris, pH 5.5, 25% w/v PEG 3350, 200 mM ammonium sulfate, and protein solution, X-ray diffractrion structure determination and analysis at 1.9 A resolution, molecular replacement	Entamoeba histolytica

Crystallization (Comment)

Organism

purified recombinant EhENO with two 2-phospho-D-glycerate substrate molecules bound at the two active sites, the protein solution contains 7.5 mg/ml protein in 100 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM MgCl2, sitting-drop vapour-diffusion, 20°C, mixing of equal volumes of precipitant solution, contraining 100 mM Bis-Tris, pH 5.5, 25% w/v PEG 3350, 200 mM ammonium sulfate, and protein solution, X-ray diffractrion structure determination and analysis at 1.9 A resolution, molecular replacement

Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Entamoeba histolytica	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	C4LXE8	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged ENO from Escherichia coli BL21 (DE3) by glutathione affinity chromatography, cleavage of the GST tag, and gel filtration	Entamoeba histolytica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Entamoeba histolytica	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	substrate binding in pre-catalytic state and during catalysis, recombinant enzyme, overview	Entamoeba histolytica	phosphoenolpyruvate + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	in the crystal structure of 2-phospho-D-glycerate-complexed enzyme, ENO forms an asymmetric dimer with one active site in the open conformation and the other active site in the closed conformation, overview	Entamoeba histolytica

Synonyms

Synonyms	Comment	Organism
EhENO	-	Entamoeba histolytica
enolase	-	Entamoeba histolytica