Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
F137L/E363G | the dimer-dimer interface mutant destabilizes the octameric structure, the double mutant is more easily dissociated in the presence of NaClO4 than is the wild type | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaClO4 | enolase at 19.4 mM after incubation in 0.2 M NaClO4, has 32% of its original activity and is 21% octameric. Following a 24 h dialysis against buffer, the protein is 77% octameric and has 74% of its original activity | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
452000 | - |
calculated molecular mass | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Streptococcus pyogenes | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | sedimentation velocity analysis | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Streptococcus pyogenes |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
60 | - |
mutant enzyme F137L/E363G, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2 | Streptococcus pyogenes | NaClO4 | |
200 | - |
wild type enzyme, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2 | Streptococcus pyogenes | NaClO4 |