Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of a plant like pentapeptide insert 104EWGWS108 in a highly conserved surface loop of the protein results in about 100fold decrease in kcat/Km and causes dissociation of dimeric form into monomers | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2-phospho-D-glycerate | recombinant, dimeric enzyme | Plasmodium falciparum | |
0.04 | - |
2-phospho-D-glycerate | recombinant, His-tagged, hexameric enzyme | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose beads chromatography, gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Plasmodium falciparum | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44.7 | - |
dimeric recombinant enolase shows two transitions which corresponds to dissociation of dimer in to monomer (Tm of 44.7°C) | Plasmodium falciparum |
58.1 | - |
dimeric recombinant enolase shows two transitions which corresponds to unfolding of monomer (Tm of 58.1°C) | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.6 | - |
2-phospho-D-glycerate | recombinant, dimeric enzyme | Plasmodium falciparum | |
22.2 | - |
2-phospho-D-glycerate | recombinant, His-tagged, hexameric enzyme | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
520 | - |
2-phospho-D-glycerate | recombinant, dimeric enzyme | Plasmodium falciparum | |
560 | - |
2-phospho-D-glycerate | recombinant, His-tagged, hexameric enzyme | Plasmodium falciparum |