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Literature summary for 4.2.1.11 extracted from

  • Vora, H.K.; Shaik, F.R.; Pal-Bhowmick, I.; Mout, R.; Jarori, G.K.
    Effect of deletion of a plant like pentapeptide insert on kinetic, structural and immunological properties of enolase from Plasmodium falciparum (2009), Arch. Biochem. Biophys., 485, 128-138.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information deletion of a plant like pentapeptide insert 104EWGWS108 in a highly conserved surface loop of the protein results in about 100fold decrease in kcat/Km and causes dissociation of dimeric form into monomers Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
0.04
-
2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
gel filtration Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose beads chromatography, gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Plasmodium falciparum phosphoenolpyruvate + H2O
-
r

Subunits

Subunits Comment Organism
homodimer gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
enolase
-
Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44.7
-
dimeric recombinant enolase shows two transitions which corresponds to dissociation of dimer in to monomer (Tm of 44.7°C) Plasmodium falciparum
58.1
-
dimeric recombinant enolase shows two transitions which corresponds to unfolding of monomer (Tm of 58.1°C) Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.6
-
2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
22.2
-
2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
520
-
2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
560
-
2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum