Literature summary for 4.2.1.11 extracted from

Entelis, N.; Brandina, I.; Kamenski, P.; Krasheninnikov, I.A.; Martin, R.P.; Tarassov, I.
A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae (2006), Genes Dev., 20, 1609-1620.

Search for more literature for 4.2.1.11

Application

Application	Comment	Organism
molecular biology	studies on mitochondrial import machinery of Saccharomyces cerevisiae	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	engineered enolase forms disrupted in catalytic activity retain features to direct mitochondrial import of tRNA	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	up to 7% of enolase activity associated with mitochondria, targeting towards the mitochondrial outer membrane, affinity to the mitochondrial surface more than 10fold higher for Eno2p than for Eno1p	Saccharomyces cerevisiae	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	gel electrophoresis	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Saccharomyces cerevisiae	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	W303	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	additional and independent function beyond glycolytic enzyme function, involvement in mitochondrial tRNA targeting, depletion of enolase inhibits tRNA import in vivo, activity of enolase as an alternative molecular chaperone suggested	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Saccharomyces cerevisiae	phosphoenolpyruvate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
enolase	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
additional information	specific affinity to tRNA imported into mitochondria, involved to direct mitochondrial tRNA import in vitro	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)