Literature summary for 4.2.1.11 extracted from

Hosaka, T.; Meguro, T.; Yamato, I.; Shirakihara, Y.
Crystal structure of Enterococcus hirae enolase at 2.8 A resolution (2003), J. Biochem., 133, 817-823.

Search for more literature for 4.2.1.11

Crystallization (Commentary)

Crystallization (Comment)	Organism
using the hanging drop method followed by recrystallization under the same conditions, X-ray structure solved by molecular replacement	Enterococcus hirae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	two magnesium ions per subunit are required for catalytic activity	Enterococcus hirae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46220	-	calculated from amino-acid sequence	Enterococcus hirae
46620	-	determined by mass spectrometry	Enterococcus hirae

Organism

Organism	UniProt	Comment	Textmining
Enterococcus hirae	Q8GR70	gram-positive bacteria, ATCC9790	-

Purification (Commentary)

Purification (Comment)	Organism
partial, using ammonium sulfate fractionation and gel filtration chromatography	Enterococcus hirae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
124	-	pH 7.5, 22ºC	Enterococcus hirae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Enterococcus hirae	phosphoenolpyruvate	-	r

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Release 2023.1 (January 2023)