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Literature summary for 4.2.1.11 extracted from

  • Derbise, A.; Song, Y.P.; Parikh, S.; Fischetti, V.A.; Pancholi, V.
    Role of the C-terminal lysine residues of streptococcal surface enolase in Glu- and Lys-plasminogen-binding activities of group A streptococci (2004), Infect. Immun., 72, 94-105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain XL1-Blue and BL-21 with N-terminal His-tag Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
DELTA434-435 mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
K434L/K435L mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
K435L mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
additional information replacement of the C-terminal lysine residues by leucine reduces Glu- and Lys-plasminogen-binding properties Streptococcus pyogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
Streptococcus pyogenes 9986
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Streptococcus pyogenes
-
phosphoenolpyruvate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
type M6 strain D471
-
Streptococcus pyogenes
-
M6 strain D471
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus pyogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
his-tagged recombinant protein has the same activity than the wild type Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Streptococcus pyogenes phosphoenolpyruvate
-
r
2-phospho-D-glycerate
-
Streptococcus pyogenes phosphoenolpyruvate + H2O
-
r

Synonyms

Synonyms Comment Organism
SEN
-
Streptococcus pyogenes
streptococcal surface enolase
-
Streptococcus pyogenes
streptococcal surface enolase SEN Streptococcus pyogenes