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Literature summary for 4.2.1.11 extracted from
- Purification and comparative characterization of an enolase from spinach (1984), Plant Physiol., 74, 834-840.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Solanum tuberosum | |
| additional information | contains 23 half-cystines per mol of subunit | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | - |
Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
2-phospho-D-glycerate | - |
Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 14% of the activation with Mg2+ | Spinacia oleracea | |
| Cu2+ | 20% of the activiation with Mg2+ | Spinacia oleracea | |
| Mg2+ | activation by several divalent cation, Mg2+ is most effective | Spinacia oleracea | |
| Mn2+ | 19% of the activation with Mg2+ | Spinacia oleracea | |
| Zn2+ | 12% of the activation with Mg2+ | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Spinacia oleracea |
| 93000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Spinacia oleracea | phosphoenolpyruvate | - |
? | |
| 2-phospho-D-glycerate | - |
Solanum tuberosum | phosphoenolpyruvate | - |
? | |
| 2-phospho-D-glycerate | - |
Spinacia oleracea | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | - |
Spinacia oleracea |
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Release 2023.1 (January 2023)
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