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Literature summary for 4.2.1.11 extracted from
- Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties (1974), Biochim. Biophys. Acta, 365, 285-300.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
2-phospho-D-glycerate | muscle enzyme | Rattus norvegicus |  |
| 0.14 | - |
2-phospho-D-glycerate | liver enzyme | Rattus norvegicus | |
| 0.31 | - |
phosphoenolpyruvate | liver enzyme | Rattus norvegicus | |
| 0.37 | - |
phosphoenolpyruvate | muscle enzyme | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, muscle enzyme, SDS-PAGE | Rattus norvegicus |
| 53000 | - |
2 * 53000, liver enzyme, SDS-PAGE | Rattus norvegicus |
| 91000 | - |
liver enzyme, gel filtration | Rattus norvegicus |
| 93000 | - |
muscle enzyme, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver enzyme | Rattus norvegicus |
| muscle enzyme | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | enolase I | Rattus norvegicus | - |
| blood | enolase I | Rattus norvegicus | - |
| heart | enolase I and III | Rattus norvegicus | - |
| kidney | enolase I | Rattus norvegicus | - |
| liver | enolase I | Rattus norvegicus | - |
| muscle | contains only enolase III | Rattus norvegicus | - |
| spleen | enolase I | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 87 | - |
muscle enolase | Rattus norvegicus |
| 120 | - |
muscle enolase | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Rattus norvegicus | phosphoenolpyruvate | - |
r | |
| 2-phospho-D-glycerate | - |
Rattus norvegicus | phosphoenolpyruvate + H2O | - |
? | |
| phosphoenolpyruvate | - |
Rattus norvegicus | 2-phospho-D-glycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, liver enzyme, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 49000, muscle enzyme, SDS-PAGE | Rattus norvegicus |
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