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Literature summary for 4.2.1.105 extracted from

  • Akashi, T.; Aoki, T.; Ayabe, S.
    Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis (2005), Plant Physiol., 137, 882-891.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.105

Cloned(Commentary)

Cloned (Comment) Organism
-
 Glycyrrhiza echinata
-
 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycine max
0.058
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
0.114
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycine max
0.17
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycine max
0.21
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
0.304
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata

Organism

Organism UniProt Comment Textmining
Glycine max Q5NUF3 expressed in Escherichia coli
-
Glycyrrhiza echinata Q5NUF4 expressed in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5,7,4'-tetrahydroxyisoflavanone
-
 Glycine max genistein + H2O
-
 ?
2,5,7,4'-tetrahydroxyisoflavanone activity is 0.5% of the activity with 2,7-dihydroxy-4'-methoxyisoflavanone Glycyrrhiza echinata genistein + H2O
-
 ?
2,7,4'-trihydroxyisoflavanone activity is 0.5% of the activity with 2,7-dihydroxy-4'-methoxyisoflavanone Glycyrrhiza echinata daidzein + H2O
-
 ?
2,7,4'-trihydroxyisoflavanone activity is 40% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max daidzein + H2O
-
 ?
2,7-dihydroxy-4'-methoxyisoflavanone
-
 Glycyrrhiza echinata formononetin + H2O
-
 ?
2,7-dihydroxy-4'-methoxyisoflavanone activity is 15% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max formononetin + H2O
-
 ?
p-nitrophenyl butyrate activity is 0.6% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone Glycine max ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
0.19
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
1.6
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycine max
5.3
-
 2,7,4'-trihydroxyisoflavanone pH 7.5, 30°C Glycine max
9.8
-
 2,7-dihydroxy-4'-methoxyisoflavanone pH 7.5, 30°C Glycyrrhiza echinata
18.1
-
 2,5,7,4'-tetrahydroxyisoflavanone pH 7.5, 30°C Glycine max