Literature summary for 4.2.1.104 extracted from

Schlachter, C.R.; Klapper, V.; Wybouw, N.; Radford, T.; Van Leeuwen, T.; Grbic, M.; Chruszcz, M.
Structural characterization of a eukaryotic cyanase from Tetranychus urticae (2017), J. Agric. Food Chem., 65, 5453-5462 .

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Application

Application	Comment	Organism
drug development	cyanase is potentially an attractive protein target for the development of acaricides	Tetranychus urticae

Cloned(Commentary)

Cloned (Comment)	Organism
gene tetur28g02430, DNA and amino acid sequence determination and analysis, genomic and transcriptomic analysis, phylogenetic analysis, recombinant expression of MBP-tagged or N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)	Tetranychus urticae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant MBP-tagged enzyme, sitting drop vapor diffusion method, 13 mg/ml protein solution is mixed with crystallization solution, containing 0.1 M Tris, pH 7.5, 15% w/v PEG 6000, in a 1:1 ratio, about 2 months, room temperature, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement using the crystal structure of Escherichia coli cyanase (PDB ID 2IV1) as the starting model	Tetranychus urticae

Crystallization (Comment)

Organism

purified recombinant MBP-tagged enzyme, sitting drop vapor diffusion method, 13 mg/ml protein solution is mixed with crystallization solution, containing 0.1 M Tris, pH 7.5, 15% w/v PEG 6000, in a 1:1 ratio, about 2 months, room temperature, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement using the crystal structure of Escherichia coli cyanase (PDB ID 2IV1) as the starting model

Tetranychus urticae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	recombinant monomeric His-tagged enzyme, gel filtration	Tetranychus urticae
670000	-	recombinant decameric MBP-tagged enzyme, gel filtration	Tetranychus urticae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cyanate + HCO3- + 2 H+	Tetranychus urticae	-	NH3 + 2 CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Tetranychus urticae	T1KZQ3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, recombinant MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, dialysis in presence of 5 mm maltose, ultrafiltration, and gel filtration	Tetranychus urticae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyanate + HCO3- + 2 H+	-	Tetranychus urticae	NH3 + 2 CO2	-	?

Subunits

Subunits	Comment	Organism
homodecamer	10 * 60000, about, recombinant His-tagged enzyme, SDS-PAGE	Tetranychus urticae
More	prokaryotic and eukaryotic cyanases all form homodecamers and have conserved active site residues, but display different surface areas between homodimers in the overall decameric structure. Monomer and dimer interfaces analysis, overview	Tetranychus urticae

Synonyms

Synonyms	Comment	Organism
107368746	-	Tetranychus urticae
tetur28g02430	-	Tetranychus urticae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Tetranychus urticae

General Information

General Information	Comment	Organism
evolution	genomic and transcriptomic analysis, phyloegentic analysis, the cyanase gene originates from a single horizontal gene transfer event, which precedes subsequent speciation, comparison of prokaryotic cyanases to eukaryotic cyanase from Tetranychus urticae, which all form homodecamers and have conserved active site residues, but display different surface areas between homodimers in the overall decameric structure	Tetranychus urticae
additional information	the enzyme binds specifically to DNA	Tetranychus urticae
physiological function	cyanase catalyzes the detoxification of cyanate	Tetranychus urticae