Crystallization (Comment) | Organism |
---|---|
mutant enzymes E86A and E86Q, sitting drop vapor diffusion method, using 0.2 M sodium chloride and 20% (w/v) PEG 3350 (unliganded structure), 0.19 M sodium chloride and 17.9% (w/v) PEG 3350 (cocrystallized E86A-3-dehydroshikimate complex), 0.1 M MIB buffer (pH 9.0) and 25% (w/v) PEG 1500 (soaked E86A-3-dehydroshikimate complex) | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
E86A | the mutant retains catalytic proficiency but is optimally active at more acidic conditions than the wild type enzyme (pH 5.7).The mutation reduces kcat 17fold | Salmonella enterica |
E86Q | the mutant retains catalytic proficiency and is optimally active at wild type conditions. The E86Q mutation rather modestly affects kcat, decreasing it by a factor of 4 | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
3-dehydroquinate | mutant enzyme E86Q, at pH 7.5 and 37°C | Salmonella enterica | |
0.046 | - |
3-dehydroquinate | mutant enzyme E86A, at pH 7.5 and 37°C | Salmonella enterica | |
0.053 | - |
3-dehydroquinate | wild type enzyme, at pH 7.5 and 37°C | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroquinate | Salmonella enterica | - |
3-dehydroshikimate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | P58687 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroquinate | - |
Salmonella enterica | 3-dehydroshikimate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHQD | - |
Salmonella enterica |
type I dehydroquinate dehydratase | - |
Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
3-dehydroquinate | mutant enzyme E86A, at pH 7.5 and 37°C | Salmonella enterica | |
78 | - |
3-dehydroquinate | mutant enzyme E86Q, at pH 7.5 and 37°C | Salmonella enterica | |
310 | - |
3-dehydroquinate | wild type enzyme, at pH 7.5 and 37°C | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Salmonella enterica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
3-dehydroquinate | mutant enzyme E86A, at pH 7.5 and 37°C | Salmonella enterica | |
2600 | - |
3-dehydroquinate | mutant enzyme E86Q, at pH 7.5 and 37°C | Salmonella enterica | |
5800 | - |
3-dehydroquinate | wild type enzyme, at pH 7.5 and 37°C | Salmonella enterica |