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Literature summary for 4.2.1.10 extracted from

  • Ratia, K.; Light, S.H.; Antanasijevic, A.; Anderson, W.F.; Caffrey, M.; Lavie, A.
    Discovery of selective inhibitors of the Clostridium difficile dehydroquinate dehydratase (2014), PLoS ONE, 9, e89356.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridioides difficile

Inhibitors

Inhibitors Comment Organism Structure
2,5-dichloro-N-(4-phenyl-1,3-thiazol-2-yl)benzenesulfonamide
-
Clostridioides difficile
2,5-dichloro-N-(6-methoxy-1,3-benzothiazol-2-yl)benzenesulfonamide
-
Clostridioides difficile
2-[(4,5,6,7-tetrahydro-1-benzothiophen-3-ylcarbonyl)amino]benzoic acid
-
Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydroquinate Clostridioides difficile
-
3-dehydroshikimate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap HP Ni Sepharose column chromatography, and S-200 gel filtration Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroquinate
-
Clostridioides difficile 3-dehydroshikimate + H2O
-
?

Synonyms

Synonyms Comment Organism
aroD
-
Clostridioides difficile
dehydroquinate dehydratase
-
Clostridioides difficile
DHQD
-
Clostridioides difficile

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.031
-
in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2-[(4,5,6,7-tetrahydro-1-benzothiophen-3-ylcarbonyl)amino]benzoic acid
0.034
-
in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2,5-dichloro-N-(4-phenyl-1,3-thiazol-2-yl)benzenesulfonamide
0.035
-
in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2,5-dichloro-N-(6-methoxy-1,3-benzothiazol-2-yl)benzenesulfonamide