Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
expressed in Escherichia coli BL21(DE3) cells | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
D88N | the mutant shows severely reduced activity compared to the wild type enzyme and is not active at pH 7.0 but pH 8.0 | Mycobacterium tuberculosis |
N12S | the mutant shows severely reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0114 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis | |
0.0146 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis | |
0.1776 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroquinate | Mycobacterium tuberculosis | - |
3-dehydroshikimate + H2O | - |
? | |
3-dehydroquinate | Helicobacter pylori | - |
3-dehydroshikimate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography | Mycobacterium tuberculosis |
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | - |
Mycobacterium tuberculosis | ? | - |
? | |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | - |
Helicobacter pylori | ? | - |
? | |
3-dehydroquinate | - |
Mycobacterium tuberculosis | 3-dehydroshikimate + H2O | - |
? | |
3-dehydroquinate | - |
Helicobacter pylori | 3-dehydroshikimate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHQ2 | - |
Mycobacterium tuberculosis |
DHQ2 | - |
Helicobacter pylori |
Type II dehydroquinase | - |
Mycobacterium tuberculosis |
Type II dehydroquinase | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C | Mycobacterium tuberculosis | |
0.0065 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis | |
0.1542 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C | Mycobacterium tuberculosis | |
0.6 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis | |
10.5 | - |
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid | wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C | Mycobacterium tuberculosis |