Cloned (Comment) | Organism |
---|---|
gene ca6, isolation and sequencing of Danio rerio CA VI cDNA, complete with the sequence coding for the PTX domain, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of the recombinant CA VI-PTX protein in Spodoptera frugiperda Sf9 cells using the baculovirus expression system, quantitative real-time PCR | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of gene ca6 via two different antisense gene ca6-morpholino oligonucleotides-injected larvae. Zebrafish cannot swim properly in the ca6 knockdown model, in underdeveloped or deflated swim bladder in ca6 morphant larvae is observed, swimming pattern analysis of ca6 morphant and wild-type zebrafish, phenotype, overview | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | 5-acetamido-1,3,4-thiadiazole-2-sulfonamide | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
H2CO3 | carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication | Danio rerio |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
recombinant enzyme CA VI-PTX, gel filtration, light scattering, and dynamic light scattering | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Danio rerio | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | E7F742 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | four potentially N-glycosylated Asn residues (in the motif Asn-X-Ser/Thr) are all on the surface of the monomer | Danio rerio |
Purification (Comment) | Organism |
---|---|
recombinant enzyme CA VI-PTX from Spodoptera frugiperda Sf9 cells | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | highest expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
egg | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
fin | high expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
gill | - |
Danio rerio | - |
heart | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
intestine | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
kidney | - |
Danio rerio | - |
liver | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
additional information | immunohistochemic analysis of enzyme distribution in zebrafish tissues, quantitative expression analysis of ca6 mRNA in wild-type and the ca6 morphant zebrafish at different stages of development | Danio rerio | - |
pancreas | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
skin | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
spleen | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
swim bladder | very low expression level of isozyme pentraxin-carbonic anhydrase | Danio rerio | - |
tail | - |
Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Danio rerio | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 58600, recombinant enzyme CA VI-PTX, SDS-PAGE, 5 * 58107, enzyme sequence without glycans and signal peptide excluded, sequence calculation | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
CA VI | - |
Danio rerio |
CA6 | - |
Danio rerio |
pentraxin-CA VI | - |
Danio rerio |
pentraxin-carbonic anhydrase | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
890000 | - |
H2CO3 | carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication | Danio rerio |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000005 | - |
acetazolamide | recombinant enzyme, pH and temperature not specified in the publication | Danio rerio |
General Information | Comment | Organism |
---|---|---|
malfunction | swimming pattern analysis of ca6 morphant and wild-type zebrafish, mutant phenotype of ca6 knockout fish, overview | Danio rerio |
additional information | homology modeling of isozyme CA VI including PTX domain (residues 317-530) using the structure of human isozyme CA IV as template, PDB ID 3FE4. Structure comparisons | Danio rerio |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130000 | - |
H2CO3 | carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication | Danio rerio |