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Literature summary for 4.2.1.1 extracted from

  • Patrikainen, M.S.; Tolvanen, M.E.E.; Aspatwar, A.; Barker, H.R.; Ortutay, C.; Jaenis, J.; Laitaoja, M.; Hytoenen, V.P.; Azizi, L.; Manandhar, P.; Jager, E.; Vullo, D.; Kukkurainen, S.; Hilvo, M.; Supuran, C.T.; Parkkila, S.
    Identification and characterization of a novel zebrafish (Danio rerio) pentraxin-carbonic anhydrase (2017), PeerJ, 5, e4128 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ca6, isolation and sequencing of Danio rerio CA VI cDNA, complete with the sequence coding for the PTX domain, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of the recombinant CA VI-PTX protein in Spodoptera frugiperda Sf9 cells using the baculovirus expression system, quantitative real-time PCR Danio rerio

Protein Variants

Protein Variants Comment Organism
additional information knockdown of gene ca6 via two different antisense gene ca6-morpholino oligonucleotides-injected larvae. Zebrafish cannot swim properly in the ca6 knockdown model, in underdeveloped or deflated swim bladder in ca6 morphant larvae is observed, swimming pattern analysis of ca6 morphant and wild-type zebrafish, phenotype, overview Danio rerio

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide 5-acetamido-1,3,4-thiadiazole-2-sulfonamide Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5
-
H2CO3 carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication Danio rerio

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme Danio rerio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
recombinant enzyme CA VI-PTX, gel filtration, light scattering, and dynamic light scattering Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Danio rerio
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Danio rerio E7F742
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein four potentially N-glycosylated Asn residues (in the motif Asn-X-Ser/Thr) are all on the surface of the monomer Danio rerio

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme CA VI-PTX from Spodoptera frugiperda Sf9 cells Danio rerio

Source Tissue

Source Tissue Comment Organism Textmining
brain highest expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
egg very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
fin high expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
gill
-
Danio rerio
-
heart very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
intestine very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
kidney
-
Danio rerio
-
liver very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
additional information immunohistochemic analysis of enzyme distribution in zebrafish tissues, quantitative expression analysis of ca6 mRNA in wild-type and the ca6 morphant zebrafish at different stages of development Danio rerio
-
pancreas very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
skin very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
spleen very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
swim bladder very low expression level of isozyme pentraxin-carbonic anhydrase Danio rerio
-
tail
-
Danio rerio
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Danio rerio CO2 + H2O
-
r

Subunits

Subunits Comment Organism
pentamer 5 * 58600, recombinant enzyme CA VI-PTX, SDS-PAGE, 5 * 58107, enzyme sequence without glycans and signal peptide excluded, sequence calculation Danio rerio

Synonyms

Synonyms Comment Organism
CA VI
-
Danio rerio
CA6
-
Danio rerio
pentraxin-CA VI
-
Danio rerio
pentraxin-carbonic anhydrase
-
Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
890000
-
H2CO3 carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication Danio rerio

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
acetazolamide recombinant enzyme, pH and temperature not specified in the publication Danio rerio

General Information

General Information Comment Organism
malfunction swimming pattern analysis of ca6 morphant and wild-type zebrafish, mutant phenotype of ca6 knockout fish, overview Danio rerio
additional information homology modeling of isozyme CA VI including PTX domain (residues 317-530) using the structure of human isozyme CA IV as template, PDB ID 3FE4. Structure comparisons Danio rerio

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130000
-
H2CO3 carbonate dehydratase activity, recombinant enzyme, pH and temperature not specified in the publication Danio rerio