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Literature summary for 4.2.1.1 extracted from

  • Eminoglu, A.; Vullo, D.; Asik, A.; Colak, D.N.; Supuran, C.T.; Canakci, S.; Osman Belduez, A.
    Cloning, expression and biochemical characterization of a beta-carbonic anhydrase from the soil bacterium Enterobacter sp. B13 (2016), J. Enzyme Inhib. Med. Chem., 31, 1111-1118 .
    View publication on PubMed
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene can, sequence comparisons and phylogenetic analysis, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Enterobacter sp. B13

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
 Enterobacter sp. B13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information stopped flow CO2 hydrase assay method Enterobacter sp. B13

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme, the catalytic residues Asp44 and Arg46 are involved in activation of the zinc-coordinated water molecule/hydroxide ion for catalysis Enterobacter sp. B13

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Enterobacter sp. B13
-
 CO2 + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Enterobacter sp. B13 A0A0S1NK17
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by cobalt affinity chromatography and dialysis Enterobacter sp. B13

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Enterobacter sp. B13 H2CO3
-
 r
H2CO3
-
 Enterobacter sp. B13 CO2 + H2O
-
 r
additional information stopped flow CO2 hydrase assay method Enterobacter sp. B13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24950, sequence calculation, x * 25000, recombinant His-tagged enzyme, SDS-PAGE Enterobacter sp. B13

Synonyms

Synonyms Comment Organism
B13-CA
-
 Enterobacter sp. B13
beta-carbonic anhydrase
-
 Enterobacter sp. B13
CaN
-
 Enterobacter sp. B13

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Enterobacter sp. B13

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
480000
-
 CO2 pH 8.3, 20°C, recombinant His-tagged enzyme Enterobacter sp. B13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Enterobacter sp. B13

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000789
-
 acetazolamide pH 8.3, 20°C, recombinant His-tagged enzyme Enterobacter sp. B13

General Information

General Information Comment Organism
evolution evolutionary relationship analysis, phylogenetic tree, the enzyme belongs to the beta class of carbonic anhydrases, subgroup of type II beta-CAs. The enzyme B13-CA has all the features of a catalytically effective beta-CA: (i) the putative Zn(II) ligands, Cys42, Asp44, His 98 and Cys101, (ii) the catalytic dyad involved in activation of the zinc-coordinated water molecule/hydroxide ion for catalysis, represented by the Asp44-Arg46 residues, which resembles in a way the activation of the water molecule in aspartic proteases Enterobacter sp. B13

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
56000
-
 CO2 pH 8.3, 20°C, recombinant His-tagged enzyme Enterobacter sp. B13