Cloned (Comment) | Organism |
---|---|
gene ca1, encoding delta-CA, sequence comparisons, phylogenetic analysis, recombinant His-tagged enzyme expression in Escherichia coli | Conticribra weissflogii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme | Conticribra weissflogii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Conticribra weissflogii | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Conticribra weissflogii | Q5U9J1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatagraphy | Conticribra weissflogii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Conticribra weissflogii | H2CO3 | - |
r | |
H2CO3 | - |
Conticribra weissflogii | CO2 + H2O | - |
r | |
additional information | enzyme TweCA has no esterase activity with 4-nitrophenyl acetate as substrate, measurement of CO2 hydration reaction for determination of enzyme activity by stopped-flow pH-based assay with bromothymol blue as indicator | Conticribra weissflogii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
delta carbonic anhydrase | - |
Conticribra weissflogii |
delta-carbonic anhydrase | - |
Conticribra weissflogii |
delta-class CA | - |
Conticribra weissflogii |
TweCA | - |
Conticribra weissflogii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
assay at | Conticribra weissflogii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant His-tagged enzyme, pH 8.3, 40% activity remaining after 30 min, complete inactivation after 60 min | Conticribra weissflogii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130000 | - |
CO2 | measured with hydration of CO2 activity assay, pH 7.5-8.3, 0°C | Conticribra weissflogii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.3 | assay at | Conticribra weissflogii |
General Information | Comment | Organism |
---|---|---|
evolution | several genetically distinct classes of carbonic anhydrase (CAs) exist, e.g. alpha-, beta-, gamma-, delta- and zeta-, and all of them are metalloenzymes. delta-CAs are closer to the alpha-CAs than to the other classes of the enzymes, phylogenetic analysis | Conticribra weissflogii |
physiological function | carbonic anhydrases are key enzymes involved in the acquisition of inorganic carbon for photosynthesis in phytoplankton, as they catalyze efficiently the interconversion between carbon dioxide and bicarbonate | Conticribra weissflogii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33000 | - |
CO2 | measured with hydration of CO2 activity assay, pH 7.5-8.3, 0°C | Conticribra weissflogii |