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Literature summary for 4.2.1.1 extracted from

  • Wang, G.L.; Wang, Q.; Xu, Z.C.; Wang, Y.Y.; Li, J.L.
    Identification, structural characterization and expression analysis of a novel carbonic anhydrase from freshwater mussel Hyriopsis cumingii (2017), Gene, 636, 78-86 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CA3, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis Hyriopsis cumingii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme, residues His130, His132, and His155 are zinc iron binding sites Hyriopsis cumingii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Hyriopsis cumingii
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CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Hyriopsis cumingii A0A220A4U9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the HcCA3 isozyme contains four N-glycosylation sites: Asn37, Asn44, Asn195, and Asn253 Hyriopsis cumingii
phosphoprotein the HcCA3 isozyme contains five KinasePhos sites: Tyr24, Ser25, Ser220, Thr236, Thr249 and Ser343 Hyriopsis cumingii

Source Tissue

Source Tissue Comment Organism Textmining
epidermis in-situ hybridization shows that the HcCA3 is expressed in both inside and outside epidermal cells Hyriopsis cumingii
-
mantle expression in the posterior mantle pallial (pMP) is significantly higher than that in the anterior mantle pallial (aMP), and expression is low in the mantle center (MC). Expression in the aMP, pMP and MC is significantly higher in purple mussels compared with that in white mussels Hyriopsis cumingii
-
additional information the protein sequence of the enzyme contains a signal peptide composed of 19 amino acid residues (MVVATVILLLACAAVSNCV). Quantitative RT-PCR expression analysis and in-situ hybridization in mussel tissues, overview Hyriopsis cumingii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Hyriopsis cumingii CO2 + H2O
-
r

Subunits

Subunits Comment Organism
? x * 36690, isozyme CA3, sequence calculation, x * 40000, SDS-PAGE Hyriopsis cumingii

Synonyms

Synonyms Comment Organism
alpha-CA
-
Hyriopsis cumingii
alpha-carbonic anhydrase
-
Hyriopsis cumingii
CA3
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Hyriopsis cumingii
HcCA3
-
Hyriopsis cumingii

pI Value

Organism Comment pI Value Maximum pI Value
Hyriopsis cumingii sequence calculation
-
5.92

General Information

General Information Comment Organism
additional information the CA domain of the enzyme includes 7 active site residues: His93, Gln128, His130, His132, Glu142, His155 and Thr248. Residues His130, His132, and His155 are zinc iron binding sites Hyriopsis cumingii
physiological function Hyriopsis cumingii isozyme HcCA3 play a role in the formation of shell and pearl sac formation. The mantle epithelial cell of acceptor mussels sharply divide and proliferate after being stimulated and wrap the stimulus to form pearl sac gradually. Pearl sac gradually secretes pearl essence layer by layer around stimulus and form pearl ultimately. HcCA3 expression in different parts of the mantle shows different trends during pearl formation, which suggests that it plays different roles in the process Hyriopsis cumingii