Cloned (Comment) | Organism |
---|---|
gene CA3, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis | Hyriopsis cumingii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme, residues His130, His132, and His155 are zinc iron binding sites | Hyriopsis cumingii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Hyriopsis cumingii | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyriopsis cumingii | A0A220A4U9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the HcCA3 isozyme contains four N-glycosylation sites: Asn37, Asn44, Asn195, and Asn253 | Hyriopsis cumingii |
phosphoprotein | the HcCA3 isozyme contains five KinasePhos sites: Tyr24, Ser25, Ser220, Thr236, Thr249 and Ser343 | Hyriopsis cumingii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | in-situ hybridization shows that the HcCA3 is expressed in both inside and outside epidermal cells | Hyriopsis cumingii | - |
mantle | expression in the posterior mantle pallial (pMP) is significantly higher than that in the anterior mantle pallial (aMP), and expression is low in the mantle center (MC). Expression in the aMP, pMP and MC is significantly higher in purple mussels compared with that in white mussels | Hyriopsis cumingii | - |
additional information | the protein sequence of the enzyme contains a signal peptide composed of 19 amino acid residues (MVVATVILLLACAAVSNCV). Quantitative RT-PCR expression analysis and in-situ hybridization in mussel tissues, overview | Hyriopsis cumingii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Hyriopsis cumingii | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36690, isozyme CA3, sequence calculation, x * 40000, SDS-PAGE | Hyriopsis cumingii |
Synonyms | Comment | Organism |
---|---|---|
alpha-CA | - |
Hyriopsis cumingii |
alpha-carbonic anhydrase | - |
Hyriopsis cumingii |
CA3 | - |
Hyriopsis cumingii |
HcCA3 | - |
Hyriopsis cumingii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hyriopsis cumingii | sequence calculation | - |
5.92 |
General Information | Comment | Organism |
---|---|---|
additional information | the CA domain of the enzyme includes 7 active site residues: His93, Gln128, His130, His132, Glu142, His155 and Thr248. Residues His130, His132, and His155 are zinc iron binding sites | Hyriopsis cumingii |
physiological function | Hyriopsis cumingii isozyme HcCA3 play a role in the formation of shell and pearl sac formation. The mantle epithelial cell of acceptor mussels sharply divide and proliferate after being stimulated and wrap the stimulus to form pearl sac gradually. Pearl sac gradually secretes pearl essence layer by layer around stimulus and form pearl ultimately. HcCA3 expression in different parts of the mantle shows different trends during pearl formation, which suggests that it plays different roles in the process | Hyriopsis cumingii |