Cloned (Comment) | Organism |
---|---|
gene CpsCAgamma, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) (Arctic Express DE3 competent cells), subcloning in Escherichia coli strain DH5alpha | Colwellia psychrerythraea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | sulfonamide CA inhibitor (CAI) | Colwellia psychrerythraea | |
B4O72- | - |
Colwellia psychrerythraea | |
CF3SO3- | - |
Colwellia psychrerythraea | |
Cl- | - |
Colwellia psychrerythraea | |
CN- | - |
Colwellia psychrerythraea | |
CNO- | - |
Colwellia psychrerythraea | |
CO32- | - |
Colwellia psychrerythraea | |
CS32- | - |
Colwellia psychrerythraea | |
Et2NCS2- | - |
Colwellia psychrerythraea | |
FSO3- | - |
Colwellia psychrerythraea | |
H2NSO2NH2 | - |
Colwellia psychrerythraea | |
H2NSO3H | - |
Colwellia psychrerythraea | |
HCO3- | - |
Colwellia psychrerythraea | |
HS- | - |
Colwellia psychrerythraea | |
HSO3- | - |
Colwellia psychrerythraea | |
I- | - |
Colwellia psychrerythraea | |
additional information | perchlorate, tetrafluoroborate, azide, fluoride, and bromide are not inhibitory for the enzyme | Colwellia psychrerythraea | |
NH(SO3)22- | - |
Colwellia psychrerythraea | |
NO2- | - |
Colwellia psychrerythraea | |
NO3- | - |
Colwellia psychrerythraea | |
P2O74- | - |
Colwellia psychrerythraea | |
PF6- | - |
Colwellia psychrerythraea | |
Ph-AsO3H2 | - |
Colwellia psychrerythraea | |
Ph-B(OH)2 | - |
Colwellia psychrerythraea | |
ReO4- | - |
Colwellia psychrerythraea | |
RuO4- | - |
Colwellia psychrerythraea | |
S2O82- | - |
Colwellia psychrerythraea | |
SCN- | - |
Colwellia psychrerythraea | |
SeCN- | - |
Colwellia psychrerythraea | |
SeO42- | - |
Colwellia psychrerythraea | |
SnO32- | - |
Colwellia psychrerythraea | |
SO42- | - |
Colwellia psychrerythraea | |
TeO42- | - |
Colwellia psychrerythraea | |
V2O74- | - |
Colwellia psychrerythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4700 | - |
CO2 | pH 8.3, 22°C | Colwellia psychrerythraea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme. The three amino acid residues Asn73, Gln75 and Asn202, which constitute the triad involved in the network of hydrogen bonds with the zinc-coordinated water molecule, are conserved in all the sequences of gamma-CA enzymes. The enzyme CpsCA trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis | Colwellia psychrerythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
recombinant enzyme, native PAGE | Colwellia psychrerythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | Colwellia psychrerythraea | - |
H2CO3 | - |
r | |
CO2 + H2O | Colwellia psychrerythraea 34H / ATCC BAA-681 | - |
H2CO3 | - |
r | |
H2CO3 | Colwellia psychrerythraea | - |
CO2 + H2O | - |
r | |
H2CO3 | Colwellia psychrerythraea 34H / ATCC BAA-681 | - |
CO2 + H2O | - |
r | |
additional information | Colwellia psychrerythraea | the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | ? | - |
? | |
additional information | Colwellia psychrerythraea 34H / ATCC BAA-681 | the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | Q47YG3 | i.e. Vibrio psychroerythus | - |
Colwellia psychrerythraea 34H / ATCC BAA-681 | Q47YG3 | i.e. Vibrio psychroerythus | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography | Colwellia psychrerythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Colwellia psychrerythraea | H2CO3 | - |
r | |
CO2 + H2O | - |
Colwellia psychrerythraea 34H / ATCC BAA-681 | H2CO3 | - |
r | |
H2CO3 | - |
Colwellia psychrerythraea | CO2 + H2O | - |
r | |
H2CO3 | - |
Colwellia psychrerythraea 34H / ATCC BAA-681 | CO2 + H2O | - |
r | |
additional information | the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | Colwellia psychrerythraea | ? | - |
? | |
additional information | the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons | Colwellia psychrerythraea 34H / ATCC BAA-681 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 20000, recombinant enzyme, SDS-PAGE | Colwellia psychrerythraea |
More | cross-sectional profiles of the gamma-CA trimer reveal that each monomer resembles an equilateral triangle, which is stabilized by hydrogen bonds, salt bridges and hydrophobic interactions. The trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis | Colwellia psychrerythraea |
Synonyms | Comment | Organism |
---|---|---|
CpsCA | - |
Colwellia psychrerythraea |
CpsCAgamma | - |
Colwellia psychrerythraea |
gamma-CA | - |
Colwellia psychrerythraea |
gamma-carbonic anhydrase | - |
Colwellia psychrerythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Colwellia psychrerythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
600000 | - |
CO2 | pH 8.3, 22°C | Colwellia psychrerythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
assay at | Colwellia psychrerythraea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000502 | - |
acetazolamide | pH 8.3, 22°C | Colwellia psychrerythraea | |
0.008 | - |
H2NSO2NH2 | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
0.026 | - |
Ph-B(OH)2 | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
0.037 | - |
H2NSO3H | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
0.038 | - |
Ph-AsO3H2 | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
0.58 | - |
Et2NCS2- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
1.4 | - |
CO32- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
1.7 | - |
HCO3- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
1.8 | - |
SnO32- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
2.5 | - |
CNO- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
3.3 | - |
SCN- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
3.8 | - |
CS32- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
4.3 | - |
FSO3- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
4.4 | - |
CN- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
4.5 | - |
S2O82- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
4.7 | - |
B4O72- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
4.8 | - |
HS- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
5.1 | - |
TeO42- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
5.6 | - |
PF6- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
5.8 | - |
V2O74- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
6 | - |
SeCN- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
7.3 | - |
P2O74- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
7.7 | - |
CF3SO3- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
7.8 | - |
ReO4- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
7.9 | - |
RuO4- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
7.9 | - |
NH(SO3)22- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
8.3 | - |
SeO42- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
8.7 | - |
I- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
8.8 | - |
NO2- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
9 | - |
Cl- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
9.4 | - |
SO42- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
21.7 | - |
NO3- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea | |
37.5 | - |
HSO3- | pH 8.3, 22°C, inhibition of CO2 hydration activity | Colwellia psychrerythraea |