Literature summary for 4.2.1.1 extracted from

Iqbal, S.; Saleem, M.; Azim, M.K.; Taha, M.; Salar, U.; Khan, K.M.; Perveen, S.; Choudhary, M.I.
Carbohydrazones as new class of carbonic anhydrase inhibitors synthesis, kinetics, and ligand docking studies (2017), Bioorg. Chem., 72, 89-101 .

Search for more literature for 4.2.1.1

Application

Application	Comment	Organism
drug development	discovery and development of carbonic anhydrase inhibitors is crucial for their clinical use as antiepileptic, diurectic, and antiglaucoma agents	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	carbohydrazones as another class of carbonic anhydrase inhibitors, synthesis, kinetics, and ligand docking studies, overview. Single crystal X-ray diffraction, structural coordinates of bovine carbonic anhydrase (PDB ID 1V9E) is used for docking of carbohydrazone compounds, three-dimensional structure analysis. No inhibition by N'',N'''-bis[(E)-(4-chlorophenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2-fluorophenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-phenylmethylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2-hydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(3-hydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(4-hydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2,3-dihydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2,5-dihydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(5-bromo-2-hydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2,4,6-trihydroxyphenyl)methylidene]carbonicdihydrazide, N'',N'''-bis[(E)-(2,3,4-trihydroxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2-methoxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(3-methoxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(4-methoxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2-hydroxy-5-methoxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(2-hydroxy-3-methoxyphenyl)methylidene]carbonic dihydrazide, N'',N'''-bis[(E)-(3-ethoxy-2-hydroxyphenyl)methylidene]carbonic dihydrazide, and N'',N'''-bis[(E)-(3,4-dimethoxyphenyl)methylidene]carbonic dihydrazide	Bos taurus
N'',N'''-Bis[(E)-(2,4-dichlorophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(2,6-dichlorophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(2-bromophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(2-chloro-5-nitrophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(2-chlorophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(2-nitrophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(4-bromophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(4-fluorophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
N'',N'''-bis[(E)-(4-nitrophenyl)methylidene]carbonic dihydrazide	-	Bos taurus
zonisamide	-	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	required, metalloenzyme	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Bos taurus	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00921	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	purified bovine erythrocyte CA-II	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2CO3	-	Bos taurus	CO2 + H2O	-	r
additional information	enzyme activity assay with 4-nitrophenyl acetate as substrate using the enzyme's esterase activity	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
CA-II	-	Bos taurus
carbonic anhydrase II	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bos taurus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00186	-	pH 7.4, 25°C	Bos taurus	zonisamide

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Release 2023.1 (January 2023)