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Literature summary for 4.2.1.1 extracted from
- Biochemical properties of a novel and highly thermostable bacterial alpha-carbonic anhydrase from Sulfurihydrogenibium yellowstonense YO3AOP1 (2012), J. Enzyme Inhib. Med. Chem., 27, 892-897.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Sulfurihydrogenibium yellowstonense |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
CO2 | Vmax: 7100 WAU/mg protein, pH 7.6, 0°C | Sulfurihydrogenibium yellowstonense |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
SDS-PAGE | Sulfurihydrogenibium yellowstonense |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurihydrogenibium yellowstonense | - |
- |
- |
| Sulfurihydrogenibium yellowstonense YO3AO81 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Sulfurihydrogenibium yellowstonense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + H2O | - |
Sulfurihydrogenibium yellowstonense | H2CO3 | - |
? | |
| CO2 + H2O | - |
Sulfurihydrogenibium yellowstonense YO3AO81 | H2CO3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-carbonic anhydrase | - |
Sulfurihydrogenibium yellowstonense |
| SspCA | - |
Sulfurihydrogenibium yellowstonense |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
assay at | Sulfurihydrogenibium yellowstonense |
| 95 | - |
optimum temperature | Sulfurihydrogenibium yellowstonense |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
enzyme is active and stable for up to 50 h | Sulfurihydrogenibium yellowstonense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Sulfurihydrogenibium yellowstonense |
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Release 2023.1 (January 2023)
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