Literature summary for 4.2.1.1 extracted from

Lehneck, R.; Neumann, P.; Vullo, D.; Elleuche, S.; Supuran, C.T.; Ficner, R.; Poeggeler, S.
Crystal structures of two tetrameric beta-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora (2014), FEBS J., 281, 1759-1772.

Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a His-tagged fusion protein in Escherichia coli	Sordaria macrospora

Crystallization (Commentary)

Crystallization (Comment)	Organism
determined to a resolution of 2.7A	Sordaria macrospora

Inhibitors

Inhibitors	Comment	Organism	Structure
acetazolamide	-	Sordaria macrospora
bromide	-	Sordaria macrospora
chloride	-	Sordaria macrospora
perchlorate	-	Sordaria macrospora
phenylarsonic acid	-	Sordaria macrospora
phenylboronic acid	-	Sordaria macrospora
sulfamate	-	Sordaria macrospora
sulfamide	-	Sordaria macrospora
sulfate	-	Sordaria macrospora
tetrafluoroborate	-	Sordaria macrospora

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Sordaria macrospora	5829	-
mitochondrion	contain N-terminal signal peptide for translocation into mitochondria	Sordaria macrospora	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25100	-	calculated from cDNA	Sordaria macrospora
25900	-	calculated from cDNA	Sordaria macrospora

Organism

Organism	UniProt	Comment	Textmining
Sordaria macrospora	C1L335	-	-
Sordaria macrospora	C1L336	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Sordaria macrospora	H2CO3	-	?

Subunits

Subunits	Comment	Organism
tetramer	crystal structure	Sordaria macrospora

Synonyms

Synonyms	Comment	Organism
beta-carbonic anhydrase	-	Sordaria macrospora
CAS1	-	Sordaria macrospora
CAS2	-	Sordaria macrospora

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Sordaria macrospora

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
12000	-	CO2	20°C, pH 8.3	Sordaria macrospora
13000	-	CO2	20°C, pH 8.3	Sordaria macrospora

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.3	-	assay at	Sordaria macrospora

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000445	-	acetazolamide	20°C, pH 8.3	Sordaria macrospora
0.000816	-	acetazolamide	20°C, pH 8.3	Sordaria macrospora
0.009	-	phenylboronic acid	20°C, pH 8.3	Sordaria macrospora
0.035	-	phenylarsonic acid	20°C, pH 8.3	Sordaria macrospora
0.048	-	sulfamide	20°C, pH 8.3	Sordaria macrospora
0.054	-	phenylarsonic acid	20°C, pH 8.3	Sordaria macrospora
0.056	-	phenylboronic acid	20°C, pH 8.3	Sordaria macrospora
0.069	-	sulfamate	20°C, pH 8.3	Sordaria macrospora
0.072	-	sulfamate	20°C, pH 8.3	Sordaria macrospora
0.084	-	sulfamide	20°C, pH 8.3	Sordaria macrospora
4.8	-	sulfamide	20°C, pH 8.3	Sordaria macrospora
9.2	-	chloride	20°C, pH 8.3	Sordaria macrospora
9.3	-	bromide	20°C, pH 8.3	Sordaria macrospora
100	-	chloride	value above, 20°C, pH 8.3	Sordaria macrospora
100	-	bromide	value above, 20°C, pH 8.3	Sordaria macrospora

General Information

General Information	Comment	Organism
malfunction	enzyme complements Saccharomyces cerevisiae deletion mutant Nce103	Sordaria macrospora

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1210	-	CO2	20°C, pH 8.3	Sordaria macrospora
1300	-	CO2	20°C, pH 8.3	Sordaria macrospora

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Release 2023.1 (January 2023)