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Literature summary for 4.2.1.1 extracted from
- Purification and characterization of an extracellular carbonic anhydrase from Pseudomonas fragi (2009), Process Biochem., 44, 1293-1297.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetazolamide | - |
Pseudomonas fragi |  |
| As3+ | 37.1% residual activity at 1 mM | Pseudomonas fragi | |
| azide | - |
Pseudomonas fragi | |
| bromide | - |
Pseudomonas fragi | |
| Chlorate | - |
Pseudomonas fragi | |
| chloride | - |
Pseudomonas fragi | |
| cyanide | - |
Pseudomonas fragi | |
| EDTA | 71.49% residual activity at 1 mM | Pseudomonas fragi | |
| fluoride | - |
Pseudomonas fragi | |
| Hg2+ | 40.9% residual activity at 1 mM | Pseudomonas fragi | |
| Iodide | - |
Pseudomonas fragi | |
| nitrate | - |
Pseudomonas fragi | |
| Pb2+ | 46.6% residual activity at 1 mM | Pseudomonas fragi | |
| sulfanilamide | - |
Pseudomonas fragi | |
| sulfate | - |
Pseudomonas fragi | |
| thiocyanate | - |
Pseudomonas fragi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas fragi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 106.6% relative activity at 1 mM | Pseudomonas fragi | |
| Fe2+ | 102.6% relative activity at 1 mM | Pseudomonas fragi | |
| additional information | not influenced by Cu2+, K+, Na+, Ca2+, and Mg2+ | Pseudomonas fragi | |
| Zn2+ | 109.25% relative activity at 1 mM | Pseudomonas fragi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
3 * 31000, calculated from the log plot obtained from the relative mobility of the standard proteins in SDS-PAGE | Pseudomonas fragi |
| 94000 | - |
gel filtration | Pseudomonas fragi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fragi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation and 4-aminomethylbenzenesulfonamide agarose column chromatography | Pseudomonas fragi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the crude supernatant displays a specific activity of 2.44 U/mg, the 4.52fold purified enzyme shows a specific activity of 11.03 U/mg, at 25°C | Pseudomonas fragi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Pseudomonas fragi | 4-nitrophenol + acetate | - |
? | |
| CO2 + H2O | - |
Pseudomonas fragi | H2CO3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 31000, calculated from the log plot obtained from the relative mobility of the standard proteins in SDS-PAGE | Pseudomonas fragi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonic anhydrase | - |
Pseudomonas fragi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pseudomonas fragi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | stable at 35-45°C, there is a progressive decline in the stability of enzyme following increase in temperature from 35°C to 55°C, the enzyme retains 100% activity at 35°C while 89% and 86% activity is retained at 40°C and 45°C respectively. In contrast, 21.0% residual activity is observed at 55°C following 1 h incubation at the respective temperatures | Pseudomonas fragi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas fragi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | the enzyme retains 100% activity at pH 8.0 while more than 80% enzyme activity is retained in the pH range 7.0-8.5 after 24 h incubation at room temperature, 45% and 65% enzyme activity at pH 5.0 and 10.0 respectively | Pseudomonas fragi |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
- |
Pseudomonas fragi | acetazolamide | |
| 0.0008 | - |
- |
Pseudomonas fragi | cyanide | |
| 0.003 | - |
- |
Pseudomonas fragi | azide | |
| 0.007 | - |
- |
Pseudomonas fragi | sulfanilamide | |
| 1.5 | - |
- |
Pseudomonas fragi | thiocyanate | |
| 29 | - |
- |
Pseudomonas fragi | Iodide | |
| 35 | - |
- |
Pseudomonas fragi | nitrate | |
| 69 | - |
- |
Pseudomonas fragi | bromide | |
| 180 | - |
- |
Pseudomonas fragi | chloride | |
| 200 | - |
- |
Pseudomonas fragi | sulfate | |
| 240 | - |
- |
Pseudomonas fragi | Chlorate | |
| 350 | - |
- |
Pseudomonas fragi | fluoride | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas fragi | the enzyme is induced in presence of CaCO3 | up |
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