Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
Nce103 in complex with a substrate analog at 2.04 A resolution, hanging drop vapor diffusion method, using 20% (w/v) PEG4000, 0.1 M sodium citrate, pH 5.6, 0.1 M sodium acetate, 20% (v/v) ethylene glycol, at 16°C | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncated Nce103 without the sequence coding for the N-terminal 13 residues Nce103DELTAN13 shows almost the same activity as the full length Nce103, whereas the activity of the truncated Nce103 without the sequence coding for the N-terminal 50 residues Nce103DELTAN50 is completely abolished | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc ion is coordinated by the three highly conserved residues Cys57, His112 and Cys115 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53615 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and Superdex 75 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Saccharomyces cerevisiae | H2CO3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
carbonic anhydrase | - |
Saccharomyces cerevisiae |
Nce103 | - |
Saccharomyces cerevisiae |