Literature summary for 4.2.1.1 extracted from

Teng, Y.B.; Jiang, Y.L.; He, Y.X.; He, W.W.; Lian, F.M.; Chen, Y.; Zhou, C.Z.
Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103 (2009), BMC Struct. Biol., 9, 67.

Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
Nce103 in complex with a substrate analog at 2.04 A resolution, hanging drop vapor diffusion method, using 20% (w/v) PEG4000, 0.1 M sodium citrate, pH 5.6, 0.1 M sodium acetate, 20% (v/v) ethylene glycol, at 16°C	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	the truncated Nce103 without the sequence coding for the N-terminal 13 residues Nce103DELTAN13 shows almost the same activity as the full length Nce103, whereas the activity of the truncated Nce103 without the sequence coding for the N-terminal 50 residues Nce103DELTAN50 is completely abolished	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	a zinc ion is coordinated by the three highly conserved residues Cys57, His112 and Cys115	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P53615	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity column chromatography and Superdex 75 gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Saccharomyces cerevisiae	H2CO3	-	r

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
carbonic anhydrase	-	Saccharomyces cerevisiae
Nce103	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)