Literature summary for 4.2.1.1 extracted from

Di Fiore, A.; Monti, S.M.; Hilvo, M.; Parkkila, S.; Romano, V.; Scaloni, A.; Pedone, C.; Scozzafava, A.; Supuran, C.T.; De Simone, G.
Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide (2009), Proteins, 74, 164-175.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
isozyme CA XIII in the unbound state and in complex with acetazolamide, hanging drop vapour diffusion method, in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM dithiothreitol with 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
acetazolamide	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
13.8	-	CO2	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains Zn2+	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
GSTrap resin chromatography and NHS-activated Sepharose 4 Fast Flow resin chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Homo sapiens	H2CO3	-	r

Synonyms

Synonyms	Comment	Organism
CA XIII	-	Homo sapiens
carbonic anhydrase XIII	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
150000	-	CO2	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000016	-	acetazolamide	-	Homo sapiens

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Release 2023.1 (January 2023)